Escherichia coli O157:H7 esinemine Eesti piimafarmides ja veiseliha tootmise ahelas aastatel 2005–2014
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Escherichia coli O157:H7 human infections are mostly associated with consumption of raw or undercooked beef and raw milk. Ruminants, especially cattle are the main reservoir of the pathogen. The main aim of the present study was to evaluate the occurrence of E. coli O157:H7 in cattle dairy farm and beef production level in Estonia. It was found that E. coli O157:H7 is present at both dairy farm and slaughterhouse level. The occurrence of the pathogen at Estonian dairy farm level was 1.2% from 1312 cattle's sampled. At slaughterhouse level 744 hide swabs were taken from which 30 (4.0%) were positive to E. coli O157:H7. Both stx1 and stx2 genes were determined from isolates and often the combination of both genes was found to be present. Minimal inhibitory concentration was determined for 30 E. coli O157:H7 isolates which revealed 26.7% of multiresistance isolates. In 2009–2010 in total of 188 beef samples were analysed, which all were negative for O157:H7.