AFLP-analysis of genetic diversity in soybean [Glycine max (l.) Merr.] cultivars Russian and foreign selection

Abstract

Genetic diversity of 11 cultivars of soybean (Glycine max) from a VIR (N.I. Vavilov Institute of Plant Genetic Resources) collection was analyzed by the AFLP (amplified fragment length polymorphism) technique. From 18 tested primer combinations, both of them were selected for further analysis. From these two primer combinations (E32/M60 and E32/M59), 90 DNA fragments were obtained, 39 (43%) of them were polymorphic. Unique DNA fragments were found in accessions SibNIISHoz 6, Soer-4, Determinant. The Jaccard's similarity indices varied from 0.79 (between Sonata and Ugra) to 0.94 (between Ugra and Garmoniya) with an average of 0.775. Cluster analysis placed the cultivars into the several groups but separation in groups was not related to their origin or biological characteristics, even though cultivars Nordik and Soer-4 (cultivars recommended for Central Black Earth region of Russia) clustered together on the dendrogram. The obtained high values of Jaccard genetic similarity index and low level of polymorphism and differentiation illustrated a relatively low genetic diversity in our studied cultivars, which correlated with different other studies on soybean genetic diversity with AFLP-analysis.