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Campylobacter spp. and Listeria monocytogenes in poultry products in Estonia

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Date
2016
Author
Praakle, Kristi
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Abstract
Campylobacteriosis, caused by thermotolerant Campylobacter species, is continued to be one of the most commonly reported zoonosis in humans in the European Union, and Campylobacter is recognized as the most common causative agents of bacterial gastroenteritis in developed world. Listeriosis, the disease caused by Listeria monocytogenes, is a lifethreatening disease for individuals with compromised immune systems. Consumption of different foods, including poultry products, have been associated with campylobacteriosis and listeriosis. Healthy birds may shed Campylobacter spp. and L. monocytogenes in fecal material asymptomatically. Poultry meat may become contaminated with fecal matter during slaughter or through cross-contamination during processing. The main goals of the present thesis were 1) to determine the prevalence and counts of Campylobacter spp. and the prevalence of L. monocytogenes in Estonian and imported raw poultry meat, 2) to serotype and genotype using pulsed-field gel electrophoresis both Campylobacter and L. monocytogenes isolates, and 3) to determine the antimicrobial susceptibility of the Campylobacter isolates. The studies showed that the poultry products of Estonian origin and imported to Estonia were contaminated with Campylobacter spp. and L. monocytogenes. There was high sero- and genotype diversity among Campylobacter and L. monocytogenes isolates. High level of resistance to fluoroquinolones, tetracycline, ampicillin, and erythromycin among the Campylobacter isolates were detected.
 
Termofiilsed kampülobakterid on paljudes maades ühed põhilised inimese bakteriaalse mao-peensoolepõletiku põhjustajad ja Euroopa Liidu riikides on kampülobakterioos jätkuvalt kõige sagedamini esinev zoonoos. Listerioos on nakkushaigus, mille kutsub esile bakter Listeria monocytogenes ja mis ohustab eelkõige immuunpuudulikkusega inimesi. Haigusjuhtude põhjuseks on kas bakteriga Campylobacter spp. või bakteriga L. monocytogenes saastunud toiduainete, sh linnuliha, tarbimine. Linnud võivad olla nii kampülobakterite kui ka listeeriate asümptomaatilised kandjad. Peamiselt saastub linnuliha fekaalse saastumise teel lindude algtöötlemisel tapamajas või ristsaastumise teel linnuliha edaspidise töötlemise käigus. Käesoleva väitekirja peamised eesmärgid olid 1) määrata bakteri Campylobacter spp. levimus ja arvukus ning bakteri L. monocytogenes levimus Eestis toodetud ja Eestisse imporditud toores linnulihas, 2) serotüpiseerida ja genotüpiseerida pulseeriva välja geelelektroforeesi meetodil toorest linnulihast isoleeritud patogeenitüved ja 3) määrata bakteri Campylobacter spp. isolaatide tundlikkus antibiootikumide suhtes. Tehtud uuringud näitasid, et Eestis toodetud ja Eestisse imporditud linnuliha osutus saastunuks bakteritega Campylobacter spp. ja L. monocytogenes. Uuritud patogeenide tüpiseerimine viitas sero- ja genotüübilisele mitmekesisusele. Tuvastati isoleeritud kampülobakterite tüvede suur resistentsus fluorokinoloonide, tetratsükliini, ampitsilliini ja erütromütsiini suhtes.
 
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http://hdl.handle.net/10492/2462
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DSpace software copyright © 2002-2016  DuraSpace
All items in EMU digital archive DSpace are protected by original copyright, with all rights reserved, unless otherwise indicated.
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