Comparison of lycopene and β-carotene content in tomatoes determined with chemical and non-destructive methods
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Kuupäev
2019
Kättesaadav alates
Autorid
Alsina, I.
Dubova, L.
Duma, M.
Erdberga, I.
Avotiņš, A.
Rakutko, S.
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Abstrakt
Tomatoes are one of the most popular vegetables due of their wide use as food.
Tomatoes are not only tasty fruit, but one of its benefits - high carotenoids content is well-known.
Non-destructive analyses methods are used more and more in different industries. It is cheaper,
faster and environmentally friendly way of analyse than traditional chemical methods. But these
methods need references to the traditional ones. The aim of this study was to find the correlation
between lycopene and β-carotene content in tomatoes determined with reflectance spectrometer
and extraction of pigments. Content of two carotenoids (lycopene and β-carotene) was determined
in 27 varieties of tomatoes. Red, pink, orange, yellow and brown fruits were included in
experiment. Reflectance spectrums of tomatoes fruits were obtained with remote sensing portable
spectroradiometer RS-3500 (Ltd.Spectral Evolution). Tetrahydrofuran was used for extraction of
pigments. Absorption spectra of extract were obtained by spectrophotometer UV-Vis -1800 (Ltd.
Shimadzu). Linear regression analyses were performed to correlate spectral data with lycopene
and β-carotene concentrations measured by pigment extraction. The best reflectance region for
lycopene spectral detection was 570 ± 5 nm, but for β-carotene 487 ± 5 nm. Reflectance indexes
for both pigments were worked out. High linear correlation (R2 > 0.9) between spectral
parameters and lycopene concentration was detected. Correlation between results obtained with
methods used for β-carotene determination was lower and depended of colour of tomatoes fruits.
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Article
Märksõnad
Lycopersicon esculentum, reflectance spectrum, reflectance index, articles