Measuring and alleviating drought stress in pea and lentil
Laen...
Kuupäev
2023
Kättesaadav alates
Autorid
Prysiazhniuk, O.
Maliarenko, O.
Biliavska, L.
Voitovska, V.
Kononenko, L.
Klymovych, N.
Poltoretska, N.
Strilets, O.
Voievoda, L.
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Estonian University of Life Sciences
Abstrakt
Water deficit in the soil can cause drought stress in plants and drastically affect plant
growth and crop yield. Therefore, early detection of drought stress in plants followed by the
timely application of agronomic measures to alleviate plant conditions is crucial. This research
aimed to study the agronomic practices that could reduce the sensitivity of pea and lentil to
drought stress. The practices included (i) soil amendment with moisture retainer (hydrogel), (ii)
seed treatment with a growth regulator to promote root formation, (iii) application of a biological
formulation to boost soil mycorrhizal biota, and (iv) foliar application of micro fertilisers. The
research was carried out in Ukraine in 2015−2020. Drought stress in plants was detected by
measuring chlorophyll fluorescence with a portable fluorometer Floratest and calculating the ratio
of variable to maximum fluorescence Fv/Fm of the photosystem. The content of proline, high
values of which in vegetative organs point out to stress in plants, was determined by colorimetric
analysis using ninhydrin.
In pea, the incorporation of hydrogel (Aquasorb) and growth regulator (Mycofriend) combined
with seed treatment (Kelpak SC) and foliar application of micro fertiliser (Biovit or Freya-Aqua
Legumes) at BBCH 14 led to obtaining Fv/Fm values from 0.81 to 0.82. Similarly in lentil, the
maximum value of Fv/Fm (0.67) was obtained with the application of all studied agronomic
practices, with the correlation coefficient between yield and Fv/Fm at the flowering stage (BBCH
61) r = 0.97. In pea, the correlation between yield and Fv/Fm at the budding stage (BBCH 51)
was r = 0.99. The content of proline in photosynthetic plant organs was species-specific;
however, in the control treatment, where plants were exposed to drought, its maximum value was
1.10 μmol g-1 in pea and 1.40 μmol g-1 in lentil, while with the application of the proposed
agronomic practices proline content was only 0.56 μmol g-1 in pea and 0.36 μmol g-1 in lentil.
Obtained strong correlation between proline content in plant vegetative organs and the ratio of
variable to maximum fluorescence Fv/Fm of the plant photosystem indicates that measurement
of Fv/Fm with portable fluorometer might be an effective method of early identification of
drought stress in pea and lentil.
Kirjeldus
Received: January 31st, 2023 ; Accepted: April 17th, 2023 ; Published: April 22nd, 2023 ; Correspondence: ollpris@gmail.com
Märksõnad
chlorophyll fluorescence, portable fluorometer, hydrogel, growth regulator, micro fertiliser, articles