Comparison of in vitro regeneration and transformation efficiency among five vietnamese indica rice cultivars
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Kuupäev
2025
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Kirjastaja
Estonian University of Life Sciences
Abstrakt
This study optimized the in vitro regeneration and transformation processes for five popular indica rice cultivars in Vietnam named as BC15, Bac Thom 7 (BT7), Khang Dan 18 (KD18), OM5451, and TBR225. The highest callus induction rates (83–93.67%) were achieved on a medium containing 2.5 mgL-l 2,4-D, supplemented with 500 mg L-l each of L-proline, L-glutamine, and casein. Higher or lower concentrations of 2,4-D resulted in reduced callus induction. Among tested cultivars, BT7 exhibited the highest callus induction rate of 93.67%. Shoot regeneration was optimized on a medium with a combination of 0.5 mg L-l kinetin, 2.0 mg L-l BAP, and 0.5 mg L-l NAA, resulting in regeneration rates ranging from 80.23% to 90.77% across cultivars, with BT7 exhibiting the highest regeneration rate (90.77%). Hygromycin at 20 mg L-l was optimal for selecting transgenic calli, with BT7 showing the highest transformation efficiency opdetermined to be optimal for selecting transgenic calli, with BT7 achieving the highest transformation efficiency (44% hygromycin-resistant callus and 29% GUS expression). PCR analysis confirmed a high proportion (87.5%) of transgenic plants in the BT7 cultivar. These findings indicate that the BT7 is the most suitable cultivar for genetic transformation and gene editing applications.
Kirjeldus
Received: January 13th, 2025 ; Accepted: April 28th, 2025 ; Published: May 4th, 2025 ; Correspondence: dungnt@tuaf.edu.vn
Märksõnad
callus, indica rice, in vitro, regeneration, shoot, articles
Viide
Cao, L. Q., Nguyen, P. D., Pham, X. H., La, V. H., Nguyen, X. V., & Nguyen, T. D. (2025). Comparison of in vitro regeneration and transformation efficiency among five vietnamese indica rice cultivars. https://doi.org/10.15159/AR.25.033