Biosynthesis of glycosidase inhibitors on wheat bread wastes hydrolysate medium by Streptomyces sp. 170
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Kuupäev
2019
Kättesaadavus
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Abstrakt
The aim of the present study is to investigate the potential effect of bread hydrolysate
as a novel nutrient medium for cultivating Streptomyces sp. 170 (S.170). Moreover, it evaluates
the productivity and inhibitory activity of pancreatic α-amylase inhibitors (PAAI). Bread
hydrolysate medium (BHM) and corn starch hydrolysate medium (CHM) prepared with αamylase enzyme concentrations (1.5 and 2.5 units g
-1 bread) and (1.5 units g
-1
corn starch),
respectively were utilized in the study. The Seherde-Blair and modified Akulova methods were
applied to evaluate the carbohydrates concentration and the inhibitory activity of the media
respectively. Results of bread and corn media were compared to each other. Furthermore, the
activity of PAAI synthesized by S.170 was compared to other Streptomyces species. The results
showed a significant difference (P < 0.05) between the total simple sugars (glucose + maltose)
concentration produced in CHM (27.5%) and BHM prepared with α-amylase 1.5 units (45.1%).
Besides, BHM produced by α-amylase 2.5 units demonstrated the maximum total concentration
of simple sugars (49.9%). In addition, 48 h of S.170 incubation were quite enough to exhibit the
highest inhibitory activity (2,632 IU mL-1
) in BHM prepared with α-amylase 2.5 units. The
analysis demonstrated a non-significant difference in the inhibitory activity of PAAI in CMH
(1,300 IU mL-1
) and BMH with α-amylase 1.5 units (1,111 IU mL-1
). Also, compared to other
Streptomyces species, S.170 conferred highly active PAAI. In conclusion, BHM showed its
efficiency to a great extent in the cultivation of S.170 and production of PAAI with a notable high
activity.
Kirjeldus
Article
Märksõnad
bread hydrolysate medium, corn starch medium, Streptomyces sp. 170, inhibitory activity, pancreatic α-amylase inhibitors, articles