Microbiome and Culture Based Analysis of Chronic Rhinosinusitis Compared to Healthy Sinus Mucosa
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Kuupäev
2018
Kättesaadav alates
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Frontiers
Abstrakt
The role of bacteria in chronic rhinosinusitis (CRS) is still not well understood. Whole
microbiome analysis adds new aspects to our current understanding that ismainly based
on isolated bacteria. It is still unclear how the results of microbiome analysis and the
classical culture based approaches interrelate. To address this, middle meatus swabs
and tissue samples were obtained during sinus surgery in 5 patients with CRS with nasal
polyps (CRSwNP), 5 patients with diffuse CRS without nasal polyps (CRSsNP), 5 patients
with unilateral purulent maxillary CRS (upm CRS) and 3 patients with healthy sinus
mucosa. Swabs were cultured, and associated bacteria were identified. Additionally,
parts of each tissue sample also underwent culture approaches, and in parallel DNA
was extracted for 16S rRNA gene amplicon-based microbiome analysis. From tissue
samples 4.2 ± 1.2 distinct species per patient were cultured, from swabs 5.4 ± 1.6.
The most frequently cultured species from the swabs were Propionibacterium acnes,
Staphylococcus epidermidis, Corynebacterium spp. and Staphylococcus aureus. The
16S-RNA gene analysis revealed no clear differentiation of the bacterial community
of healthy compared to CRS samples of unilateral purulent maxillary CRS and
CRSwNP. However, the bacterial community of CRSsNP differed significantly from the
healthy controls. In the CRSsNP samples Flavobacterium, Pseudomonas, Pedobacter,
Porphyromonas, Stenotrophomonas, and Brevundimonas were significantly enriched
compared to the healthy controls. Species isolated from culture did not generally
correspond with the most abundant genera in microbiome analysis. Only Fusobacteria,
Parvimonas, and Prevotella found in 2 unilateral purulent maxillary CRS samples by the
cultivation dependent approach were also found in the cultivation independent approach
in high abundance, suggesting a classic infectious pathogenesis of odontogenic origin
in these two specific cases. Alterations of the bacterial community might be a more
crucial factor for the development of CRSsNP compared to CRSwNP. Further studies
are needed to investigate the relation between bacterial community characteristics and
the development of CRSsNP.
Purchase of the Illumina MiSeq was kindly supported by the EU-EFRE (European Funds for Regional Development) program and funds from the University Medicine Rostock.
Purchase of the Illumina MiSeq was kindly supported by the EU-EFRE (European Funds for Regional Development) program and funds from the University Medicine Rostock.
Kirjeldus
Märksõnad
chronic rhinosinusitis, microbiology, 16S rRNA gene sequencing, community analysis, microbiome, articles