Establishment of in vitro propagation protocol of Gerbera jamesonii Bolus ex Hook f.: explant and media selection to plantlet acclimatization
Laen...
Kuupäev
2017
Kättesaadav alates
Autorid
Winarto, Budi
Prama Yufdy, Muhammad
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Estonian Academic Agricultural Society
Abstrakt
Gerbera (Gerbera jamesonii Bolus ex Hook f.) is an
important ornamental plant commodity with high economical value in
Indonesia; however, development of the plant in larger scale
commercially is constrained by the availability of qualified-planting
materials. Conventional propagation is clearly not suitable to overcome
the problem and therefore in vitro propagation protocol is importantly
addressed. In vitro propagation protocol of G. jamesonii was
successfully established in the study. Different explants (shoot tips,
young leaves, petioles and petals) were selected and cultured on
Murashige and Skoog (MS) basalt medium containing different plant
growth regulators. Shoot tips and half-strength MS medium containing
1.5 mg l-1
thidiazuron (TDZ) and 0.25 mg l-1 N6-benzylaminopurine
(BAP) were suitable explant and initiation medium for shoot formation
with 75% explant regeneration and 5.5 shoots produced per explant.
Shoots derived from selection stage were proliferated on half-strength
MS medium supplemented with 0.25 mg l-1 BAP and resulting higher
shoot regeneration up to 7.5 shoots produced per shoot with 22.8 leaves
and 0.83 cm leaf length. Multiplication of shoots on the half-strength
MS medium fortified by 0.25 mg l-1 BAP increased gradually from the
first subculture till the fifth subculture with 9.1 shoots produced per
shoot subcultured and reduced thereafter. Shoots were then rooted on
half-strength MS medium augmented with 0.5 mg l-1 α-naphthalene
acetic acid (NAA) and 1.5 g l-1
activated charcoal (AC) and produced
2.1 roots per shoot with 2.52 cm length of roots. Well-rooted shoots were
acclimatized in a mixture of burned-rice husk and organic manure (1:1,
v/v) with 95% survival rate and 114 plantlets grew well under this
treatment.
Kirjeldus
Saabunud / Received 13.02.17 ; Aktsepteeritud / Accepted 21.04.17 ; Avaldatud veebis / Published online 28.04.17 ; Vastutav autor / Corresponding author: Budi Winarto ; e-mail: budi.winarto67@yahoo.co.id
Märksõnad
explant, basalt medium, plantlet, propagation, shoot, Gerbera jamesonii, articles