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The effect of pasteurized milk extracellular vesicles on bacterial growth

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dc.contributor.authorSapugahawatte, Dulmini Nanayakkara
dc.contributor.authorMidekessa, Getnet Balcha
dc.contributor.authorGodakumara, Kasun
dc.contributor.authorPrasadini, Madhusha
dc.contributor.authorKodithuwakku, Suranga
dc.contributor.authorRoasto, Mati
dc.contributor.authorAndronowska, Aneta
dc.contributor.authorFazeli, Alireza
dc.date.accessioned2023-11-08T12:37:59Z
dc.date.available2023-11-08T12:37:59Z
dc.date.issued2023
dc.description.abstractExtracellular vesicles (EVs) are released by cells and have a lipid bilayer structure. EVs harbor various molecules, including proteins, RNAs, and DNAs. Studies of mammalian EVs are increasingly attracting the interest of researchers; however, there are only few studies of nanoparticles in food. Milk-derived EVs can survive high temperatures and digestion process, while retaining their biological activity. This study investigated the effect of pasteurized cows’ milk derived EVs on growth of five different bacteria. Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 53868, Bacillus subtilis, Bacillus cereus and Pseudomonas aeruginosa with a concentration of 1x107 CFU/ml were separately co-cultured with pasteurized milk derived EVs (1x109 EVs/μl) in Muller Hinton broth. The bacterial growth was monitored as absorbance at 620 nm over 24 hours. Bacteria treated with phosphate buffer solution (PBS) were considered negative control throughout the experiment. The percentage bacterial growth difference was COMBIVET & OH-BOOST JOINT CONFERENCE 2023 55 determined with respect to negative control and results expressed as mean ± standard error of mean. All analyses were performed in three biological triplicates and each biological replicate consisted of three technical replicates. Co-culture of bacteria with milk EVs demonstrated that EVs could decrease the growth of S. aureus, B. subtilis, B. cereus and P. aeruginosa. Highest growth inhibition was observed for B. subtilis (33.9% ± 2.4) followed by B. cereus (18.1% ± 3.7) at 6 hours of incubation. S. aureus and P. aeruginosa growth were inhibited by 12.9% ± 1.3 and 9.9% ± 3.5 respectively after 9 hours incubation. Only the S. aureus growth inhibition at 9 hours was statistically significant (P=0.022) according to T-test. However, E. coli growth was not affected by milk EVs compared to the control. In conclusion, the dietary EVs can be absorbed by bacteria and pasteurized milk derived EVs has a selective inhibitory activity on the growth of some bacteria.eng
dc.identifier.isbn978-9916-719-30-5
dc.identifier.publicationConverging Extracellular Vesicle Research in all domains of life towards understanding their potential functional application : Proceedings of the COMBIVET & OH-BOOST Joint Conference 2023: 8-9th September 2023. Tartu, 2023eng
dc.identifier.urihttp://hdl.handle.net/10492/8676
dc.publisherEstonian University of Life Scienceseng
dc.relationThis work was supported by the Estonian Research Council grant (PRG1441).eng
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)eng
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/eng
dc.subjectpasteurized milkeng
dc.subjectextracellular vesicleseng
dc.subjectbacterial growtheng
dc.subjectconference proceedingseng
dc.subjectabstractseng
dc.titleThe effect of pasteurized milk extracellular vesicles on bacterial growtheng
dc.typeinfo:eu-repo/semantics/conferenceObjecteng

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