The effect of pasteurized milk extracellular vesicles on bacterial growth
Laen...
Kuupäev
2023
Kättesaadav alates
Autorid
Sapugahawatte, Dulmini Nanayakkara
Midekessa, Getnet Balcha
Godakumara, Kasun
Prasadini, Madhusha
Kodithuwakku, Suranga
Roasto, Mati
Andronowska, Aneta
Fazeli, Alireza
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Estonian University of Life Sciences
Abstrakt
Extracellular vesicles (EVs) are released by cells and have a lipid bilayer
structure. EVs harbor various molecules, including proteins, RNAs, and
DNAs. Studies of mammalian EVs are increasingly attracting the interest of
researchers; however, there are only few studies of nanoparticles in food.
Milk-derived EVs can survive high temperatures and digestion process,
while retaining their biological activity. This study investigated the effect
of pasteurized cows’ milk derived EVs on growth of five different bacteria.
Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 53868, Bacillus
subtilis, Bacillus cereus and Pseudomonas aeruginosa with a concentration
of 1x107 CFU/ml were separately co-cultured with pasteurized milk
derived EVs (1x109 EVs/μl) in Muller Hinton broth. The bacterial growth
was monitored as absorbance at 620 nm over 24 hours. Bacteria treated with
phosphate buffer solution (PBS) were considered negative control
throughout the experiment. The percentage bacterial growth difference was COMBIVET & OH-BOOST JOINT CONFERENCE 2023 55
determined with respect to negative control and results expressed as
mean ± standard error of mean. All analyses were performed in three
biological triplicates and each biological replicate consisted of three
technical replicates. Co-culture of bacteria with milk EVs demonstrated that
EVs could decrease the growth of S. aureus, B. subtilis, B. cereus and P.
aeruginosa. Highest growth inhibition was observed for B. subtilis (33.9%
± 2.4) followed by B. cereus (18.1% ± 3.7) at 6 hours of incubation. S. aureus
and P. aeruginosa growth were inhibited by 12.9% ± 1.3 and 9.9% ± 3.5
respectively after 9 hours incubation. Only the S. aureus growth inhibition
at 9 hours was statistically significant (P=0.022) according to T-test.
However, E. coli growth was not affected by milk EVs compared to the
control. In conclusion, the dietary EVs can be absorbed by bacteria and
pasteurized milk derived EVs has a selective inhibitory activity on the
growth of some bacteria.
Kirjeldus
Märksõnad
pasteurized milk, extracellular vesicles, bacterial growth, conference proceedings, abstracts