Improving single cell protein yields and amino acid profile via mutagenesis: review of applicable amino acid inhibitors for mutant selection
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Kuupäev
2021
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Abstrakt
Single cell protein (SCP) is a good alternative for substituting plant and animal derived
dietary proteins, since SCP production is more environmentally friendly, consumes less water,
requires smaller land areas and its effect on climate change is much less pronounced than it is in
the case of agriculturally derived proteins. Another advantage of SCP is that it is possible to use
a wide variety of biodegradable agro-industrial by-products for the cultivation of SCP producing
microorganisms. However, to make single cell protein technology more widely available and
improve its economic viability in such markets as animal and fish feed industries, it is necessary
to improve the protein yields and amino acid profiles in microorganism strains capable of using
agro-industrial by-products. One way to improve the strains used in the process is to create and
select SCP-rich mutants. In this review authors propose a novel approach to create SCP-rich
mutants with improved total protein content and essential amino acid profiles. In this approach
amino acid inhibitors are used to create selective pressure on created mutants. It is expected that
mutants with the most pronounced growth would either have higher total protein content,
increased essential amino acid concentrations or both, when cultivated on selective plates
containing one or multiple amino acid inhibitors. This paper reviews the most suitable groups of
amino acid inhibitors that could be used for selection of new strains of SCP-producing
microorganisms.
Kirjeldus
Received: February 4th, 2021 ; Accepted: April 24th, 2021 ; Published: May 18th, 2021 ; Correspondence: kriss.spalvins@rtu.lv
Märksõnad
mutagenesis, microbial protein, essential amino acids, amino acid inhibitors, herbicides, low-cost substrate, agricultural residues, articles