In search of the best technological solutions for creating edible protein-rich mutants: a multi-criteria analysis approach
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Kuupäev
2024
Kättesaadav alates
Autorid
Berzina, I.
Raita, S.
Kalnins, M.
Spalvins, K.
Kuzmika, I.
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Estonian University of Life Sciences
Abstrakt
Single-cell protein (SCP) is a promising alternative for replacing plant and animalderived dietary proteins. SCP contains essential nutrients and high levels of essential amino acids
(AA). Given the versatility of microbial strains and waste substrates that can be used as
feedstocks, many variations of production processes can be explored. Improving these
microorganism strains by enhancing their properties and productivity is vital to increasing SCP
competitiveness. One of the options to enhance microorganism strains would be by creating
mutants with better AA profiles. By using mutagenesis and AA inhibitors it should be possible
the create novel strains with improved AA-producing properties. The use of AA inhibitors to
promote selective pressure on SCP-producing strains is a novel concept and is not a widely
explored approach, therefore, the further development of this method should be explored. This
paper used a multi-criteria decision analysis method to evaluate different technological factors
vital for creating protein-rich mutants. These factors are microorganism strains, agro-industrial
waste substrates used as process feedstocks, AA inhibitors, and mutagenesis methods.
Microorganisms Candida utilis and Bacillus subtilis showed the highest potential for being used.
Molasses was the ‘closest to the ideal’ substrate to be used as feedstock for SCP production. As
the most promising mutagenesis method ethyl methane sulphonate was selected. Glufosinate
ammonium and methionine sulfoximine for both bacteria and fungi were identified as the best
inhibitors for SCP-rich mutant selection. Identified combinations of optimal solutions for
microorganisms, substrates, inhibitors, and mutagenesis techniques should be further investigated
and evaluated in laboratory settings. This could help to increase SCP's competitiveness as a
sustainable protein source.
Kirjeldus
Received: January 16th, 2024 ; Accepted: May 1st, 2024 ; Published: May 10th, 2024 ; Correspondence: Indra.Berzina@rtu.lv
Märksõnad
agro-industrial waste, amino acids, amino acid inhibitors, biomass, herbicides, lowcost substrate, microbial protein, microorganisms, multi-criteria analysis, MCDA, mutagenesis, proteins, residues, single-cell proteins, SCP, TOPSIS, waste biomass, articles