Morphological, molecular, and pathogenic characterization of Colletotrichum gloeosporioides sensu stricto associated with imported citrus fruits
Laen...
Kuupäev
2023
Kättesaadav alates
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Estonian University of Life Sciences
Abstrakt
Colletotrichum gloeosporioides is a fungal pathogen that causes anthracnose disease
in citrus fruits. C. gloeosporioides has the potential to be carried by imported citrus fruits. The
aim of this study was to detect, isolate, identify, and test the pathogenicity of C. gloeosporioides
associated with imported citrus fruits. The detection method was carried out by freezing imported
citrus fruits at -20 °C for 15 hours. C. gloeosporioides was identified morphologically and
molecularly. The morphological identification method was done by observing mycelial colonies
and conidia. The molecular identification method was done by the Polymerase Chain Reaction
technique using 3 locus of ITS, GAPDH, and TUB2. The pathogenicity was tested by attaching
1 culture circle of C. gloeosporioides to the surface of the citrus fruit and injecting 100 µl of
conidia suspension at a density of 10⁵ mL-1 into the inside of the citrus fruit. The main
results showed that C. gloeosporioides was successfully detected at 4 days after incubation by
the emergence of mycelia on the surface of citrus fruits. Morphological and molecular
identification proved that the species of fungus was C. gloeosporioides sensu stricto which was a
pathogenic fungus on imported and local citrus fruits with a disease incidence value of 100%,
and a disease severity value of 97.78% and 98.89%, respectively.
Kirjeldus
Received: March 19th, 2023 ; Accepted: August 5th, 2023 ; Published: August 24th, 2023 ; Correspondence: widodo@apps.ipb.ac.id
Märksõnad
anthracnose, disease incidence, disease severity, identification, pathogenicity, articles