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Molecular genetics analysis of milk protein gene polymorphism of dairy cows and breeding bulls in Latvia

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dc.contributor.authorSmiltina, D.
dc.contributor.authorGrislis, Z.
dc.date.accessioned2018-04-18T12:06:02Z
dc.date.available2018-04-18T12:06:02Z
dc.date.issued2018
dc.descriptionArticleeng
dc.description.abstractMilk protein is the most valuable component of milk from a dietary point of view. More than 95% of ruminants’ milk proteins are coded by six structural genes: two whey proteins (α – lactalbumin and β – lactoglobulin) and four caseins (α S1 – and α S2 – caseins, β – casein, κ – casein). The object of the research was the genetic polymorphisms of milk protein genes in populations of cows and breeding bulls of milk producing breeds in Latvia. The aim was to promote cow breeding in Latvia by developing and testing mo lecular genetics analyses for future quantity and quality analysis of the dairy cows’ population in Latvia, based on the research of genes encoding milk protein polymorphism. In methodology the molecular markers were chosen which would be suitable for char acterization of polymorphism of five milk protein genes in the population of dairy cows reared in Latvia. As a genetic method chosen the Restriction Fragment Length Polymorphism (RFLP) method and most analysed alleles of milk proteins. Using data of 719 DN A samples of dairy cows, the analysis of Latvian cows’ population was carried out through six SNP of five milk protein genes: C SN1S1 c. – 175 A > G, CSN2 – c. 4451 A > C, CSN3 c. 11625 C > T and c. 11661 A > C, LAA c.15A > G and LGB c. 3106T > C. The results of PCR - RFLP analysis showed, as it was expected, that all genotypes were found in the populations.eng
dc.identifier.issn1406-894X
dc.identifier.publicationAgronomy Research, 2018, vol. 16, no. 3, pp. 900-909eng
dc.identifier.urihttp://hdl.handle.net/10492/3904
dc.identifier.urihttp://dx.doi.org/10.15159/ar.18.084
dc.rights.holderCopyright 2009 by Estonian University of Life Sciences, Latvia University of Agriculture, Aleksandras Stulginskis University, Lithuanian Research Centre for Agriculture and Forestry. No part of this publication may be reproduced or transmitted in any form, or by any means, electronic or mechanical, incl. photocopying, electronic recording, or otherwise without the prior written permission from the Estonian University of Life Sciences, Latvia University of Agriculture, Aleksandras Stulginskis University, Lithuanian Research Centre for Agriculture and Forestryeng
dc.subjectmilk proteinseng
dc.subjectdairy cattleeng
dc.subjectpolymorphismseng
dc.subjectLatvian populationeng
dc.subjectarticleseng
dc.titleMolecular genetics analysis of milk protein gene polymorphism of dairy cows and breeding bulls in Latviaeng
dc.typeArticleeng

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2018, Vol. 16, No. 3