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Amperometric method for the determination of cellulase activity and its optimization using response surface method

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dc.contributor.authorNero, Georg
dc.contributor.authorKivirand, Kairi
dc.contributor.authorOthman, Sana Ben
dc.contributor.authorRinken, Toonika
dc.date.accessioned2024-02-27T12:02:26Z
dc.date.available2024-02-27T12:02:26Z
dc.date.issued2022
dc.description.abstractCellulases are a group of enzymes, which catalyse different steps of cellulose hydrolysis, and are broadly used in industry as unpurified mixtures of several enzymes. The total activity of cellulase is defined as the ability of the enzyme to produce glucose, which is the final product of cellulose hydrolysis, and is expressed in cellulase units. However, common strategies for the determination of the cellulolytic activity of industrial cellulase preparations are based on the assessment of different steps of cellulose hydrolysis, and the results obtained with different methods are not similar. The aim of the present study was to develop an assay for the determination of cellulase activity that relies on the amperometric determination of the final product of cellulose hydrolysis glucose. The assay conditions were optimized using response surface methodology (RSM) combined with Box-Behnken design. The detection limit of the proposed method was 1.71 ± 0.06 U. We compared the results of the amperometric method with the ones obtained with the spectrophotometric method and viscosimetry in a commercial cellulose preparation.eng
dc.description.abstractThis research was funded by the European Union’s Horizon 2020 research and innovation program project VALORTECH under Grant agreement No. 810630 and the Mobilitas Pluss ERA Chair support (No. MOBEC006).eng
dc.description.sponsorshipThis research was funded by the European Union’s Horizon 2020 research and innovation program project VALORTECH under Grant agreement No. 810630 and the Mobilitas Pluss ERA Chair support (No. MOBEC006).eng
dc.identifier.issn2093-3371
dc.identifier.publicationJournal of Analytical Science and Technology (2022) 13:21eng
dc.identifier.urihttp://hdl.handle.net/10492/8852
dc.identifier.urihttps://doi.org/10.1186/s40543-022-00331-8
dc.publisherSpringerOpeneng
dc.rightsAttribution 4.0 International (CC BY 4.0)eng
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.rights© The Author(s) 2022.eng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/eng
dc.subjectcellulase activityeng
dc.subjectamperometric detectioneng
dc.subjectresponse surface methodeng
dc.subjectoptimization of assay conditionseng
dc.subjectarticleseng
dc.titleAmperometric method for the determination of cellulase activity and its optimization using response surface methodeng
dc.typeinfo:eu-repo/semantics/articleeng

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