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Study of transhydrogenase systems features in the mutants of the yeast pachysolen tannophilus for the production of ethanol and xylitol from agricultural wastes

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dc.contributor.authorBolotnikova, O.
dc.contributor.authorBazarnova, J.
dc.contributor.authorAronova, E.
dc.contributor.authorBolotnikova, T.
dc.date.accessioned2020-06-09T09:45:21Z
dc.date.available2020-06-09T09:45:21Z
dc.date.issued2020
dc.description.abstractThe key catabolic enzymes of D-xylose, an important structural component of different agricultural wastes, were studied in cells of mutant strains of the xylose-assimilating yeast Pachysolen tannophilus. The evaluation of catalytic activity and cofactor specificity of xylose reductase (ЕС 1.1.1.307) and xylitol dehydrogenase (ЕС 1.1.1.9) confirmed the dependence of intracellular catabolic pathway for D-xy lose on the NAD×H/NADP×H ratio, formed under microaerobic conditions. The study of total activity of some NAD+ /NAP×H-dependent dehydrogenases revealed the metabolic characteristics of the yeast cells, which could ensure selective ethanol or xylitol production. Thus, the efficient involvement of D-xylose into the Embden–Meyerhof–Parnas pathway provided not only the high activities of xylose reductase and xylitol dehydrogenase, but also of 1-glycerophosphate dehydrogenase (EC 1.1.1.8) and lactate dehydrogenase (ЕС 1.1.1.27), respectively. The inhibition of activity of these enzymes led to selective production of xylitol from D-xylose. On the base of the experimental results, the principles of metabolic engineering of xylose-assimilating yeasts were formulated. The possibility of bioethanol and xylitol production from different agricultural wastes using xyloseassimilating yeasts are discussed.eng
dc.identifier.issn1406-894X
dc.identifier.publicationAgronomy Research, 2020, vol. 18, Special Issue 1, pp. 720–728eng
dc.identifier.urihttp://hdl.handle.net/10492/6029
dc.identifier.urihttps://doi.org/10.15159/ar.20.158
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) ; openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectagricultural wasteseng
dc.subjectD-xyloseeng
dc.subjectmutants of the yeast P. tannophiluseng
dc.subjectxylose reductaseeng
dc.subjectxylitol dehydrogenaseeng
dc.subject1-glycerophosphate dehydrogenaseeng
dc.subjectlactate dehydrogenaseeng
dc.subjectethanoleng
dc.subjectxylitoleng
dc.subjectarticleseng
dc.titleStudy of transhydrogenase systems features in the mutants of the yeast pachysolen tannophilus for the production of ethanol and xylitol from agricultural wasteseng
dc.typeArticleeng

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2020, Vol. 18, Special Issue 1