Study of transhydrogenase systems features in the mutants of the yeast pachysolen tannophilus for the production of ethanol and xylitol from agricultural wastes
Laen...
Kuupäev
2020
Kättesaadav alates
Autorid
Bolotnikova, O.
Bazarnova, J.
Aronova, E.
Bolotnikova, T.
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Abstrakt
The key catabolic enzymes of D-xylose, an important structural component of different
agricultural wastes, were studied in cells of mutant strains of the xylose-assimilating yeast
Pachysolen tannophilus. The evaluation of catalytic activity and cofactor specificity of xylose
reductase (ЕС 1.1.1.307) and xylitol dehydrogenase (ЕС 1.1.1.9) confirmed the dependence of
intracellular catabolic pathway for D-xy lose on the NAD×H/NADP×H ratio, formed under
microaerobic conditions. The study of total activity of some NAD+
/NAP×H-dependent
dehydrogenases revealed the metabolic characteristics of the yeast cells, which could ensure
selective ethanol or xylitol production. Thus, the efficient involvement of D-xylose into the
Embden–Meyerhof–Parnas pathway provided not only the high activities of xylose reductase and
xylitol dehydrogenase, but also of 1-glycerophosphate dehydrogenase (EC 1.1.1.8) and lactate
dehydrogenase (ЕС 1.1.1.27), respectively. The inhibition of activity of these enzymes led to
selective production of xylitol from D-xylose. On the base of the experimental results, the
principles of metabolic engineering of xylose-assimilating yeasts were formulated. The
possibility of bioethanol and xylitol production from different agricultural wastes using xyloseassimilating yeasts are discussed.
Kirjeldus
Märksõnad
agricultural wastes, D-xylose, mutants of the yeast P. tannophilus, xylose reductase, xylitol dehydrogenase, 1-glycerophosphate dehydrogenase, lactate dehydrogenase, ethanol, xylitol, articles