Fast Protein and Metabolites (Nucleotides and Nucleosides) Liquid Chromatography Technique and Chemical Sensor for the Assessment of Fish and Meat Freshness
Laen...
Kuupäev
2023
Kättesaadav alates
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
MDPI
Abstrakt
Fast protein and metabolite liquid chromatography (FPLMC) was introduced years ago to
enable the easy separation of high-molecular compounds such as proteins from small molecules and
the identification of the low-molecular substances. In this paper, the method is applied for the rapid
evaluation of freshness and monitoring the aging of animal meat and fish. A novel chromatographic
sensor was developed with a deep UV LED-based photometric detection unit (255–265 nm), an
original flow cuvette and registration scheme; the processing of a chromatogram with the sensor
takes approximately 15 min. Strict isochronism between the elution of ATP metabolites, mainly
hypoxanthine (Hx) and inosine monophosphate (IMP), and the time of maturation of meat or fish,
was discovered. A new freshness index H* = [Hx]/[IMP] was introduced, which is proportional to the
instrumental delay time in the FPMLC chromatograms: the H* index < 0.5 indicates the presence of
inosine monophosphate (IMP) and the high quality of the meat or fish. Reasonably strong correlations
were revealed between data obtained by FPMLC and total volatile basic nitrogen TVB-N (for fish) or
volatile fatty acids VFA (for meat) content. Moreover, putative nucleotide salvage and an increase in
the concentration of IMP were observed in fish after heat treatment using the FPMLC sensor and
NMR technique.
This research was partially financially supported by the Estonian Research Council grant (PRG 1441) of the Estonian University of Life Sciences (A.S., L.R., D.A., T.P., M.R.) and by DFG (Deutsche Forschungsgemeinschaft), project number 315440263, project title: “Verhalten von Proteinen in mikrofluidischen Kanälen (Behavior of proteins in microfluidic channels)” (N.M., S.H.).
This research was partially financially supported by the Estonian Research Council grant (PRG 1441) of the Estonian University of Life Sciences (A.S., L.R., D.A., T.P., M.R.) and by DFG (Deutsche Forschungsgemeinschaft), project number 315440263, project title: “Verhalten von Proteinen in mikrofluidischen Kanälen (Behavior of proteins in microfluidic channels)” (N.M., S.H.).
Kirjeldus
Märksõnad
fast protein liquid chromatography, UV detection, meat freshness, fish freshness, inosine monophosphate, hypoxanthine, nucleotide salvage, articles
Viide
Kuznetsov, A.; Frorip, A.; Sünter, A.; Kasvand, N.; Korsakov, V.; Konoplev, G.; Stepanova, O.; Rusalepp, L.; Anton, D.; Püssa, T.; et al. Fast Protein and Metabolites (Nucleotides and Nucleosides) Liquid Chromatography Technique and Chemical Sensor for the Assessment of Fish and Meat Freshness. Chemosensors 2023, 11, 69. https://doi.org/10.3390/ chemosensors11010069