Enrichment of the grains from rye wort after shock-activator-disintegrating processing
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Kuupäev
2019
Kättesaadavus
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Abstrakt
In this study, the mode of obtaining grains (pellets) from wort prepared from rye
processed by the shock-activator-disintegrator (SAD), has been developed. Additionally, the
enrichment of the grains by proteins using the strain of yeast producing proteins was carried out.
For cultivation of a pure culture of a strain of yeasts producing proteins, grains with a
concentration of 43.00 g 100 g
-1
reducing substances and 62.71 mg g
-1 of the total amount of
amino acid were used. Different concentrations (10%, 20%, 30% and 40% by weight of the
grains) of filtrate 24.90% dry matter, 21.00 g 100 g
-1
reducing substances and 10.82 mg g
-1 of the
total amount of free amino acid was added to the grains. As nitrogen and phosphorus-containing
mineral feed, diammonium phosphate was added to the nutrient medium. To obtain a pure culture
of Candida tropicalis, SK-4 yeast strain was isolated. The content of crude protein and the
concentration of amino acids were determined. The morphological state of the cells was assessed.
The results of this study show that to prepare the nutrient medium for a pure culture of a strain of
protein microorganisms-producers and its cultivation, it is necessary to add 30% of the filtrate to
the grains, while the proportion of crude protein in the protein-containing additive reaches more
than 40%. The resulting protein-enriched product has a balanced amino acid composition, which
is of interest as a protein-containing feed additive and its fine particle size distribution allows its
use for feeding farm animals.
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Märksõnad
shock-activator-disintegrator (SAD), rye filtrate and grains (pellets), proteolytic enzyme complex, Candida tropicalis, protein-enriched product, articles