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Comparative study of extraction of soy molasses isoflavones and in vivo bioconversion of daidzein into S-equol in rats models

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dc.contributor.authorSlesarev, G.P.
dc.contributor.authorGlukhareva, T.V.
dc.contributor.authorDuru, K.C.
dc.contributor.authorShevyrin, V.A.
dc.contributor.authorLyubyakina, P.N.
dc.contributor.authorKovaleva, E.G.
dc.date.accessioned2021-05-03T10:54:25Z
dc.date.available2021-05-03T10:54:25Z
dc.date.issued2021
dc.descriptionReceived: February 8th, 2021 ; Accepted: April 24th, 2021 ; Published: April 30th, 2021 ; Correspondence: grigory.slesarev@urfu.rueng
dc.description.abstractWe compared different extraction methods for isolation of isoflavones from soybean molasses. Since conventional extraction methods are time-consuming, expensive and unsustainable, we have compared them with the NADES extraction method, which does not have these disadvantages. Fermentation-assisted technique and extraction with Natural Deep Eutectic Solvents (NADES) were compared to the conventional extraction methods. Based on the results obtained, we selected the optimal technique for isoflavones isolation. Isoflavones were identified by gas chromatography equipped with mass spectrometer (GC-MS), whereas their quantities were determined using high performance liquid chromatography (HPLC). In vivo metabolism of daidzein to S-equol was performed in rats with quantification of a yield of S-equol as a result of daidzein in vivo conversion in rats’ intestines.eng
dc.identifier.issn1406-894X
dc.identifier.publicationAgronomy Research, 2021, vol. 19, Special Issue 2, pp. 1167–1178eng
dc.identifier.urihttp://hdl.handle.net/10492/6465
dc.identifier.urihttps://doi.org/10.15159/ar.21.056
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) ; openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectsoy molasseseng
dc.subjectS-equoleng
dc.subjectNADESeng
dc.subjectextractioneng
dc.subjectdaidzeineng
dc.subjectgenisteineng
dc.subjectfermentationeng
dc.subjectGC-MSeng
dc.subjectHPLCeng
dc.subjectarticleseng
dc.titleComparative study of extraction of soy molasses isoflavones and in vivo bioconversion of daidzein into S-equol in rats modelseng
dc.typeArticleeng

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2021, Vol. 19, Special Issue 2