Comparative study of extraction of soy molasses isoflavones and in vivo bioconversion of daidzein into S-equol in rats models
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Kuupäev
2021
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Kirjastaja
Abstrakt
We compared different extraction methods for isolation of isoflavones from soybean
molasses. Since conventional extraction methods are time-consuming, expensive and
unsustainable, we have compared them with the NADES extraction method, which does not have
these disadvantages. Fermentation-assisted technique and extraction with Natural Deep Eutectic
Solvents (NADES) were compared to the conventional extraction methods. Based on the results
obtained, we selected the optimal technique for isoflavones isolation. Isoflavones were identified
by gas chromatography equipped with mass spectrometer (GC-MS), whereas their quantities
were determined using high performance liquid chromatography (HPLC). In vivo metabolism of
daidzein to S-equol was performed in rats with quantification of a yield of S-equol as a result of
daidzein in vivo conversion in rats’ intestines.
Kirjeldus
Received: February 8th, 2021 ; Accepted: April 24th, 2021 ; Published: April 30th, 2021 ; Correspondence: grigory.slesarev@urfu.ru
Märksõnad
soy molasses, S-equol, NADES, extraction, daidzein, genistein, fermentation, GC-MS, HPLC, articles