Lactiplantibacillus plantarum tüvede kasutusvõimalused postbiootikumide arendamisel
Laen...
Kuupäev
2025
Kättesaadavus
04.09.2025
Autorid
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Eesti Maaülikool
Abstrakt
Postbiootikumid on mikroobse päritoluga ühendid, mis võivad peremeesorganismile pakkuda võrreldavat või suuremat füsioloogilist kasu kui probiootikumid. Toiduainetööstuses on postbiootikumidel kasutuspotentsiaal mikrobioloogilise ohutuse tagamiseks ja toidu säilivusaja pikendamiseks. Lisaks toetavad nad toidu funktsionaalseid omadusi ja kvaliteeti, olles perspektiivsed lisaained tervist edendavates toodetes.
Bakalaureusetöö eesmärk oli hinnata Lactiplantibacillus plantarum tüvede ensümaatilist aktiivsust ja ohutusprofiili, et selgitada nende sobivust kandidaattüvedena postbiootikumide arendamisel. Uuritavaks materjaliks oli kümme leivajuuretisest isoleeritud L. plantarum tüve. Tüvede ohutust hinnati hemolüütilise aktiivsuse ja antibiootikumide minimaalsete inhibeerivate kontsentratsioonide alusel. Ensümaatiline aktiivsus määrati poolkvantitatiivselt API ZYM biokeemiliste testidega.
Töö tulemustest selgus, et kõigil uuritud L. plantarum tüvedel puudus hemolüütiline aktiivsus, mis viitab nende ohutusele. Enamik mikroobitüvesid olid testitud antibiootikumide suhtes tundlikud, välja arvatud vankomütsiini ja streptomütsiini suhtes ilmnenud resistentsus, mis on liigispetsiifiline ega kujuta riski antibiootikumiresistentsuse levikuks. Erandiks oli L. plantarum tüvi 16F, millel tuvastati resistentsus erütromütsiini suhtes. Kõik uuritud tüved avaldasid kõrget aktiivsust leutsiin arüülamidaasi, valiin arüülamidaasi, β-galaktosidaasi, β-glükosidaasi ja N-atsetüül-β-glükosaminidaasi suhtes. See viitab tugevale ensümaatilisele potentsiaalile, eriti valkude ja süsivesikute metabolismi valdkonnas, mis toetab nende võimalikku kasutust seedimisprotsesside soodustamisel ning peremeesorganismi funktsionaalse tervise edendamisel.
Kokkuvõttes kinnitavad saadud tulemused, et uuritud L. plantarum tüvedest on postbiootikumide arendamiseks eelkõige sobilikud tüved 1F, 5F, 6F, 11F, 13F ja 17F, kuna need on ohutud ning omavad kõrgemat ensümaatilist aktiivsust. Edasistes uuringutes tuleks põhjalikumalt käsitleda nende ensümaatilist aktiivsust ning bioaktiivsete metaboliitide tootmist.
Postbiotics are microbially derived compounds that can provide the host organism with comparable or greater physiological benefits than probiotics. In the food industry, postbiotics have potential applications for ensuring microbiological safety and extending the shelf life of food products. Additionally, they support the functional properties and quality of foods, making them promising additives in health-promoting products. The bachelor’s thesis aimed to evaluate the enzymatic activity and safety profile of Lactiplantibacillus plantarum strains to determine their suitability as candidate strains for postbiotics development. The study material consisted of ten L. plantarum strains isolated from sourdough. The safety of the strains was assessed based on hemolytic activity and minimum inhibitory concentrations of antibiotics. Enzymatic activity was semi-quantitatively determined using API ZYM biochemical tests. The results showed that none of the tested L. plantarum strains exhibited hemolytic activity, indicating their safety. Most of the microbial strains were sensitive to the tested antibiotics, except for resistance to vancomycin and streptomycin, which is species-specific and does not pose a risk for the spread of antibiotic resistance. An exception was L. plantarum strain 16F, which showed resistance to erythromycin. All tested strains demonstrated high activity of leucine arylamidase, valine arylamidase, β-galactosidase, β-glucosidase, and N-acetyl-β-glucosaminidase. This indicates a strong enzymatic potential, especially in protein and carbohydrate metabolism, supporting their possible use in enhancing digestion processes and promoting the functional health of the host organism. In conclusion, the results confirm that among the studied L. plantarum strains, strains 1F, 5F, 6F, 11F, 13F, and 17F are particularly suitable for postbiotic development due to their safety and higher enzymatic activity. Further studies should more thoroughly address their enzymatic activity and production of bioactive metabolites.
Postbiotics are microbially derived compounds that can provide the host organism with comparable or greater physiological benefits than probiotics. In the food industry, postbiotics have potential applications for ensuring microbiological safety and extending the shelf life of food products. Additionally, they support the functional properties and quality of foods, making them promising additives in health-promoting products. The bachelor’s thesis aimed to evaluate the enzymatic activity and safety profile of Lactiplantibacillus plantarum strains to determine their suitability as candidate strains for postbiotics development. The study material consisted of ten L. plantarum strains isolated from sourdough. The safety of the strains was assessed based on hemolytic activity and minimum inhibitory concentrations of antibiotics. Enzymatic activity was semi-quantitatively determined using API ZYM biochemical tests. The results showed that none of the tested L. plantarum strains exhibited hemolytic activity, indicating their safety. Most of the microbial strains were sensitive to the tested antibiotics, except for resistance to vancomycin and streptomycin, which is species-specific and does not pose a risk for the spread of antibiotic resistance. An exception was L. plantarum strain 16F, which showed resistance to erythromycin. All tested strains demonstrated high activity of leucine arylamidase, valine arylamidase, β-galactosidase, β-glucosidase, and N-acetyl-β-glucosaminidase. This indicates a strong enzymatic potential, especially in protein and carbohydrate metabolism, supporting their possible use in enhancing digestion processes and promoting the functional health of the host organism. In conclusion, the results confirm that among the studied L. plantarum strains, strains 1F, 5F, 6F, 11F, 13F, and 17F are particularly suitable for postbiotic development due to their safety and higher enzymatic activity. Further studies should more thoroughly address their enzymatic activity and production of bioactive metabolites.
Kirjeldus
Bakalaureusetöö
Toiduainete tehnoloogia õppekaval
Märksõnad
bakalaureusetööd, piimhappebakter, bioaktiivsed ühendid, ensümaatiline aktiivsus, antibiootikumitundlikkus