Soolestiku normaalmikrofloora antibiootikumiresistentsus kliiniliselt tervetel koertel
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Kuupäev
2013
Kättesaadav alates
ainult raamatukogus, only in library
Autorid
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Abstrakt
Uurimistöö eesmärgiks oli välja selgitada kliiniliselt tervete koerte soolestiku normaalmikrofloora (Esherichia coli, Enterococcus spp.) antibiootikumiresistentsus ning leida võimalikke seoseid resistentsuse tekkimise ja antibiootikumide manustamise vahel.
Uuringus osales 86 kliiniliselt tervet koera, kes ei olnud saanud antibakteriaalset ravi viimase kolme kuu jooksul.
Soolestiku normaalmikrofloora isoleerimiseks võeti koertelt roojaproov, mis edasiseks bakterioloogiliseks analüüsiks saadeti Veterinaar- ja Toidulaboratooriumisse. Laboris isoleeriti koerte roojaproovidest E. coli ja Enterococcus spp., mida uuriti antibiootikumitundlikkusele diskdifusiooni meetodiga. E. coli antibiootikumitundlikkust hinnati ampitsilliini, tsiprofloksatsiini, nalidaksiinhape, gentamütsiini, streptomütsiini, tetratsükliini, sulfametoksasooli, trimetoprimi, klooramfenikooli, kanamütsiini, tsefatoksiimi ja tseftasidiimi suhtes. Enterococcus spp. antibiootikumitundlikkust hinnati ampitsilliini, erütromütsiini, gentamütsiini, tetratsükliini, klooramfenikooli, vankomütsiini, tsiprofloksatsiini ja linesoliidi suhtes.
Uuritud 86 koera roojaproovist isoleeriti E. coli 60 (69,7%) proovist ja Enterococcus spp. 66 (76,7%) proovist. E. coli isolaatidest 15% olid resistentsed vähemalt ühe uuritud antibiootikumi suhtes ja kolmel bakteritüvel oli arenenud multiresistentsus. Kõige enam oli E. coli resistentne ampitsilliini, streptomütsiini ja trimetoprimi suhtes. Enterococcus spp. tüvedest olid resistentsed vähemalt ühe antibiootikumi suhtes 60,6% uuritud isolaatidest. Multiresistentne oli ainult üks isolaat. Kõige rohkem esines enterokokkidel resistentsust tetratsükliini suhtes (50%), vähem tsiprofloksatsiini ja erütromütsiini suhtes. Resistentsust vankomütsiinile uuringus ei leitud. Käesolevas uuringus leiti oluline seos antibiootikumiresistentsuse ja koerte kehakaalu vahel (p < 0,05). Mida suurem oli koera kehakaal, seda suurem risk oli antibiootikumiresistentsuse arenemiseks. Olulist seost ei esinenud antibiootikumiravi saamise ja resistentsuse vahel.
The aim of the study was to find out antimicrobial resistance of normal enteric microflora (Esherichia coli, Enterococcus spp.) in clinically healthy dogs and find potential links between development of resistance and use of antibiotics. In the present study 86 clinically healthy dogs were included. The dogs were not treated with antimicrobials during the last three months.For the isolation of normal enteric microflora a sample was taken from dog’s faeces and was sent to Veterinary and Food Laboratory for further bacterial analysis. Strains of E. coli and Enterococcus spp isolated from dog faeces, were investigated for antimicrobial susceptibility using the disk diffusion method. The antimicrobial susceptibility of E. coli was tested to ampicillin, ciprofloxacin, nalidixic acid, gentamicin, streptomycin, tetracyclin, sulfamethoxazole, trimethoprim, chloramphenicol, kanamycin, cefotaxime and ceftazidime. The antimicrobial susceptibility of Enterococcus spp. was tested to ampicillin, erythromycin, gentamicin, tetracyclin, choramphenicol, vancomycin, ciprofloxacin and linezolid. A total of 86 E. coli strains were isolated from 60 (69,7%) and Enterococcus spp. from 66 (76,7%) samples. Among the E. coli isolates 15% were resistant to at least one antibiotic and three strains had developed multidrug resistance. Most of E. coli strains were resistant against ampicillin, streptomycin, sulfamethoxazole and trimethoprim. Among Enterococcus spp. isolates 60,6% were resistant to at least one antibiotic. Only one isolate was multidrug resistant. Most enterococci were resistant against tetracyclin (50%), less resistant against ciprofloxacin and erythromycin. No resistance to vancomycin was detected. In the present study there was found link between antimicrobial resistance and dog’s body weight (p < 0,05). The dog's with bigger body weight had greater risk for developing of antibiotic resistance. There was no significant association between antibiotic treatment and antibiotic resistance.
The aim of the study was to find out antimicrobial resistance of normal enteric microflora (Esherichia coli, Enterococcus spp.) in clinically healthy dogs and find potential links between development of resistance and use of antibiotics. In the present study 86 clinically healthy dogs were included. The dogs were not treated with antimicrobials during the last three months.For the isolation of normal enteric microflora a sample was taken from dog’s faeces and was sent to Veterinary and Food Laboratory for further bacterial analysis. Strains of E. coli and Enterococcus spp isolated from dog faeces, were investigated for antimicrobial susceptibility using the disk diffusion method. The antimicrobial susceptibility of E. coli was tested to ampicillin, ciprofloxacin, nalidixic acid, gentamicin, streptomycin, tetracyclin, sulfamethoxazole, trimethoprim, chloramphenicol, kanamycin, cefotaxime and ceftazidime. The antimicrobial susceptibility of Enterococcus spp. was tested to ampicillin, erythromycin, gentamicin, tetracyclin, choramphenicol, vancomycin, ciprofloxacin and linezolid. A total of 86 E. coli strains were isolated from 60 (69,7%) and Enterococcus spp. from 66 (76,7%) samples. Among the E. coli isolates 15% were resistant to at least one antibiotic and three strains had developed multidrug resistance. Most of E. coli strains were resistant against ampicillin, streptomycin, sulfamethoxazole and trimethoprim. Among Enterococcus spp. isolates 60,6% were resistant to at least one antibiotic. Only one isolate was multidrug resistant. Most enterococci were resistant against tetracyclin (50%), less resistant against ciprofloxacin and erythromycin. No resistance to vancomycin was detected. In the present study there was found link between antimicrobial resistance and dog’s body weight (p < 0,05). The dog's with bigger body weight had greater risk for developing of antibiotic resistance. There was no significant association between antibiotic treatment and antibiotic resistance.
Kirjeldus
Märksõnad
koer, soolestiku normaalmikrofloora, E. coli, Enterococcus spp., antibiootikumid, resistentsus, magistritööd