Molecular discovery of new allele associated with loose smut resistance gene Ut-X in spring wheat
Laen...
Kuupäev
2021
Kättesaadav alates
Autorid
Draz, I.S.
Darwish, A.K.
Abou-Elseoud, M.S.
Elassal, A.A.
Komeil, D.A.
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Abstrakt
Genes of resistance to loose smut incited by the fungus Ustilago tritici (Pers.) Rostr.
are still unknown in the Egyptian spring wheat. Loose smut incidence (LSI) was assessed in ten
wheat cultivars through a two-year field trial during 2018–2020. All of the tested cultivars
exhibited various percentages of susceptibility (> 10–70% LSI) to the disease except cultivar
Misr-3 which exhibited resistance. The most susceptible cultivars were Sakha-93 (60%),
Giza-168 (42.1%), and Misr-2 (34.28%). However, the resistant cultivar Misr-3 recorded the least
LSI amounting to 5%. The wheat cultivars were screened by the SCAR marker (Xcrc4.2) to
identify the presence/absence of loose smut resistance gene Ut-X. Molecular data revealed that
the SCAR marker (Xcrc4.2) generated two alleles in cultivars with PCR fragments size of
800-bp and ≃200-bp. The favorable allele 800-bp was generated only in the resistant Egyptian
cultivar ‘Misr-3’ and the resistant check cultivar ‘Biggar’, indicating the presence of the gene.
Meanwhile, another allele ≃ 200-bp was generated in seven Egyptian cultivars, Giza-168,
Giza-171, Misr2, Sakha-93, Gemmeiza-12, N-95, and Shandweel-1, indicating the absence of the
resistant gene. This is the first study to report resistance genes to loose smut in Egyptian spring
wheat, by detecting Ut-X in cultivar Misr-3. In addition, the study documented the first report of
another allele ≃200-bp associated with SCAR marker (Xcrc4.2). Findings also revealed that the
race-specific resistance gene Ut-X confers effective resistance to local U. tritici races, including
race T10 which could be widely incorporated in breeding programs to control the disease.
Kirjeldus
Received: February 1st, 2021 ; Accepted: April 24th, 2021 ; Published: April 29th, 2021 ; Correspondence: dr.ibrahim_draz@yahoo.com
Märksõnad
SCAR marker, resistance genes, Triticum aestivum, Ustilago tritici, Ut-X alleles, articles