Development of metabolic engineering approaches to regulate the content of total phenolics, antiradical activity and organic acids in callus cultures of the highbush blueberry (Vaccinium corymbosum L.)
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Kuupäev
2020
Kättesaadav alates
Autorid
Ramata-Stunda, A.
Valkovska, V.
Borodušķis, M.
Livkiša, D.
Kaktiņa, E.
Silamiķele, B.
Borodušķe, A.
Pentjušs, A.
Rostoks, N.
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Abstrakt
Blueberry (Vaccinium corymbosum L.) is increasingly cultivated to produce high
quality berries for consumption and potential applications in medicine, nutrition and as industrial
precursors. Seasonal availability sets limitations on chemical compound isolation from cultivated
plants. Biotechnological solutions, such as tissue cultures and metabolic engineering, can provide
sufficient amounts of plant material with reasonably high metabolite levels, which may be
adjusted by different strategies. Here, we describe our approach to modifying total phenolic
content (TPC), antiradical activity (ARA) and amounts of selected organic acids in in vitro
cultures of two varieties of V. corymbosum by varying the growth media. TPC, ARA and acid
levels were determined in mature leaves of field-grown plants and in stable callus cultures derived
from leaves of varieties ‘Bluecrop’ and ‘Duke’ grown on Murashige-Skoog (MS) and Woody
plant (WP) media supplemented with varying concentrations and combinations of different plant
growth hormones. TPC varied from 83 mg g
-1 dry weight (DW) to 142 mg g
-1 DW in leaves of
‘Bluecrop’ and ‘Duke’, respectively, and correlated with their ARA with ‘Duke’ at the lead. For
callus cultures the highest ARA, as well as the highest TPC of 94 mg g
-1 DW was observed in
‘Bluecrop’ grown on WP medium with 2,4-dichlorophenoxyacetic acid (2,4-D). High level of
quinic acid was found in the mature leaves of all tested varieties, while callus cultures exhibited
relative increase in amounts of malic, succinic and citric acids instead. Oxalic acid was found
only in callus cultures.
Kirjeldus
Märksõnad
blueberry, Vaccinium corymbosum, total phenolic content, antiradical activity, organic acids, callus cultures, articles