Boosting the monitoring of phytoplankton in optically complex coastal waters by combining pigment-based chemotaxonomy and in situ radiometry
Laen...
Kuupäev
2019
Kättesaadav alates
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Elsevier
Abstrakt
Research about the occurrence and extent of the cyanobacterial blooms in the Baltic Sea is critical due to their
increased magnitude and frequency. Monitoring of the blooms is complicated due to their spatially and tem-
porally heterogeneous nature. For adequate assessment of the water quality, phytoplankton dynamics needs to
be tracked in large areas with high monitoring frequency. The main objectives of this study were (1) to describe
phytoplankton community composition by pigment-based chemotaxonomy and validate the results with mi-
croscopy; (2) to improve the retrieval of information about phytoplankton community by combining remote
sensing with laboratory based approaches (3) to develop a region-specific algorithm to calculate cyanobacteria
biomass from reflectance spectra; (4) to detect and quantify potentially toxic bloom-forming cyanobacteria with
molecular methods. In our study the reflectance-based chlorophyll a (Chl a) values overestimated the High-
performance Liquid Chromatography (HPLC) values although the correlations with HPLC Chl a measurements
were very strong (rp ∼ 0.8, p < 0.001). We found that 709 nm/620 nm reflectance ratio correlated strongly
(rp = 0.75, p < 0.01) to cyanobacteria wet biomass in CDOM-rich Väinameri even at low cyanobacterial bio-
mass levels. Correlations between pigment-based chemotaxonomy and microscopy were significant in case of
cyanobacteria (rp = 0.73, p < 0.01), cryptophytes (rp = 0.71, p < 0.05) and dinoflagellates (rp = 0.64,
p < 0.05).
This work was supported by Estonian Ministry of Education and Research (IUT 21-02), Estonian Science Foundation (ETF9102, ETF8576), Estonian Research Council (PUTJD719), base-financed pro- ject P180023PKKH of Estonian University of Life Sciences and by Estonian Doctoral School of Earth Sciences and Ecology. We thank Dr Teele Ligi (University of Tartu) for valuable help, Simon Wright and other developers of CHEMTAX program (Australian Antarctic Division, CSIRO). The authors acknowledge CYANOCOST-COST ES 1105 for networking and sharing knowledge.
This work was supported by Estonian Ministry of Education and Research (IUT 21-02), Estonian Science Foundation (ETF9102, ETF8576), Estonian Research Council (PUTJD719), base-financed pro- ject P180023PKKH of Estonian University of Life Sciences and by Estonian Doctoral School of Earth Sciences and Ecology. We thank Dr Teele Ligi (University of Tartu) for valuable help, Simon Wright and other developers of CHEMTAX program (Australian Antarctic Division, CSIRO). The authors acknowledge CYANOCOST-COST ES 1105 for networking and sharing knowledge.
Kirjeldus
Märksõnad
reflectance, marine optics, CHEMTAX, Baltic Sea, quantitative PCR, Cyanobacteria, articles