Nõgesest, kortslehest ja teelehest eraldatud endofüütsed seened
Laen...
Kuupäev
2013
Kättesaadav alates
ainult raamatukogus, only in library
Autorid
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Abstrakt
Töö eesmärgiks oli ravimtaimedest endofüütsete seente eraldamine puhaskultuuri, nende kirjeldamine ja määramine. Uuritavad taimed olid suur teeleht (Plantago major), kõrvenõges (Urtica dioica) ja harilik kortsleht (Alchemilla vulgaris).
Endofüütide vähese uurituse tõttu on võimalik leida uusi mikroorganisme elusate taimede seesmistest kudedest. Endofüüte on järjest rohkem hakatud uurima, seoses nende suure osatähtsusega nii looduslikes kooslustes, põllumajanduses kui meditsiinis. Käesolevas töös loodeti leida uusi seenetüvesid ravimtaimedest, mida edaspidises töös saaks kasutada haigustõrjeks.
Uurimusliku töö tulemusena eraldati 85 puhaskultuuri, millede identifitseerimiseks mõõdeti nende morfoloogilisi tunnuseid (sh koniidi ja hüüfi suurused, kasvukiirused, kultuuri värvus) ning eraldati sekveneerimiseks ITS rDNA. Selle alusel määrati 12 kultuuri: Lewia infectoria, Hypoxylon howeanum, Fusarium culmorum, Apodus deciduus, Leptosphaeria sp, Pleosporales sp, Colletotrichum clavatum, Hypoxylon fuscum, Colletotrichum clavatum, Phoma multirostrata (2), Phoma sp (2).
Edaspidi tahetakse uurida, kumb toodab inimesele kasulikku bioaktiivset ainet, kas seen või ravimtaim ning kas erinevatel taimedel on erinevad endofüütsed seened.
The aim of this research was to isolate endophytic fungi to pure culture, to describe and determine them. The studied plants were broadleaf plantain (Plantago major), stinging nettle (Urtica dioica) and lady's mantle (Alchemilla vulgaris). Due to the fact that endophyte-related research is quite new, it is possible to discover new microorganisms in inner tissues of plants. The research on endophytes has been growing over time, because of their relative importance to natural community, agriculture and medicine. This research is looking for fungal strains from herbal plants, which could be used for disease control in further work. As a result, 85 pure cultures were isolated from plants, which were identified by measuring their morphological features (including conidia and hypha sizes, growth rate, pure culture colour) and separating ITS rDNA for sequencing. On this basis the 12 culture: Lewia infectoria, Hypoxylon howeanum, Fusarium culmorum, Apodus deciduus, Leptosphaeria sp, Pleosporales sp, Colletotrichum clavatum, Hypoxylon fuscum, Colletotrichum clavatum, Phoma multirostrata (2), Phoma sp (2). In the future, researchers want to find out whether fungi or herbs produce benefitial bioactive substance for people, and do different plants have different endophytic fungi.
The aim of this research was to isolate endophytic fungi to pure culture, to describe and determine them. The studied plants were broadleaf plantain (Plantago major), stinging nettle (Urtica dioica) and lady's mantle (Alchemilla vulgaris). Due to the fact that endophyte-related research is quite new, it is possible to discover new microorganisms in inner tissues of plants. The research on endophytes has been growing over time, because of their relative importance to natural community, agriculture and medicine. This research is looking for fungal strains from herbal plants, which could be used for disease control in further work. As a result, 85 pure cultures were isolated from plants, which were identified by measuring their morphological features (including conidia and hypha sizes, growth rate, pure culture colour) and separating ITS rDNA for sequencing. On this basis the 12 culture: Lewia infectoria, Hypoxylon howeanum, Fusarium culmorum, Apodus deciduus, Leptosphaeria sp, Pleosporales sp, Colletotrichum clavatum, Hypoxylon fuscum, Colletotrichum clavatum, Phoma multirostrata (2), Phoma sp (2). In the future, researchers want to find out whether fungi or herbs produce benefitial bioactive substance for people, and do different plants have different endophytic fungi.
Kirjeldus
Märksõnad
endofüütsed seened, Plantago major, Urtica dioica, Alchemilla vulgaris, ITS rDNA, bakalaureusetööd