Some features of cultivating different Chamaenerion angustifolium (L.) Scop. forms in vitro
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Kuupäev
2021
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Ajakirja pealkiri
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Kirjastaja
Estonian University of Life Sciences
Abstrakt
Chamaenerion angustifolium (L.) Scop. characterized by a wide range of economically
useful properties. White-flowered form of Ch. angustifolium (L.) Scop. Is extremely rare in
nature. At the same time, it is promising as a source of biologically active substances and as a
highly decorative plant. The optimal way to reproduce this form is clonal micropropagation.
Methods for obtaining Ch. angustifolium in vitro were developed, as well as the optimal selection
timing of starting material for micropropagation was determined. In addition, the effect of
a mineral composition of nutrient medium and plant growth regulators on the regeneration of
microshoots was studied. The highest values of morphometric parameters were achieved on
MS medium (Murashige & Skoog, 1962) supplemented with 0.5 mg L-1 BAP. The multiplication
factor of the lilac–flowered form was 8.4 ± 0.2, of the white-flowered form - 9.2 ± 0.6.
Comparative analysis of morphometric parameters during cultivation of Ch. angustifolium
showed no significant difference between the lilac-flowered and white-flowered forms. The effect
of antioxidants on the growth and development of regenerants has been shown. The most optimal
nutrient medium for clonal micropropagation of the lilac-flowered form was MS medium
(Murashige & Skoog, 1962) containing 0.5 mg L-1 of BAP, 50 mg L-1 of ascorbic acid and
50 mg L-1 of citric acids and for micropropagation of the white–flowered form it was the medium,
containing 100 mg L-1 PVP.
Kirjeldus
Received: June 9th, 2021 ; Accepted: November 8th, 2021 ; Published: November 15th, 2021 ; Correspondence: greenpro2007@rambler.ru
Märksõnad
clonal micropropagation, phenolic exudation, plant growth regulators, fireweed, articles