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Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra

dc.contributor.authorSünter, Alar
dc.contributor.authorKuznetsov, Artur
dc.contributor.authorRausepp, Piret
dc.contributor.authorPüssa, Tõnu
dc.contributor.authorToom, Lauri
dc.contributor.authorKonoplev, Georgii
dc.contributor.authorStepanova, Oksana S.
dc.contributor.authorStepanova, Oksana V.
dc.contributor.authorLyalin, Daniil
dc.contributor.authorFrorip, Aleksandr
dc.contributor.authorRoasto, Mati
dc.date.accessioned2023-11-07T11:09:50Z
dc.date.available2023-11-07T11:09:50Z
dc.date.issued2023
dc.description.abstractDietary nucleotides and nucleosides, primarily inosine monophosphate (IMP) and the adenine nucleotide pool (ANP), are widely considered as essential nutrients responsible for multiple biological functions. Food prepared from meat and fish is the main source of these substances in the human diet, and it is extremely important to implement storage and processing techniques ensuring their maximum preservation and even accumulation during maturation or conditioning. In experiments with freshly refrigerated grass carp and defrosted Alaska pollock fillets it was discovered, initially using Fast Protein and Metabolites Liquid Chromatography and the ATP- bioluminescence test, and afterwards validated by NMR spectroscopy, that heat treatment identical to conventional culinary processing in aqueous or wet media at temperatures above 62 ◦C leads to nucleotide salvage (recovery) in aged fish. A significant increase in the concentration of IMP, and even an emergence of ANP substances, were reliably demonstrated in fish samples which had already partially or fully lost these components during prolonged storage due to the ATP breakdown metabolic reactions. Owing to this recovery, the nutritive value of ready-to-eat food can be higher than was initially evaluated in raw products before heat treatment: an effect that should certainly be considered in practical nutrition. Moreover, it is necessary to reconsider the widely acknowledged system of indices of freshness based on nucleotides and nucleosides elaborated a long time ago for raw meat and fish products.eng
dc.description.abstractThis research was partially financially supported by the Estonian Research Council grant (PRG 1441) of the Estonian University of Life Sciences (A.S., P.R., T.P., M.R.).eng
dc.description.sponsorshipThis research was partially financially supported by the Estonian Research Council grant (PRG 1441) of the Estonian University of Life Sciences (A.S., P.R., T.P., M.R.).eng
dc.identifier.citationSünter, A.; Kuznetsov, A.; Raudsepp, P.; Püssa, T.; Toom, L.; Konoplev, G.; Stepanova, O.S.; Stepanova, O.V.; Lyalin, D.; Frorip, A.; et al. Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra. AppliedChem 2023, 3, 334–349. https://doi.org/10.3390/ appliedchem3020021eng
dc.identifier.issn2673-9623
dc.identifier.publicationAppliedChem 2023, 3, 334–34eng
dc.identifier.urihttp://hdl.handle.net/10492/8669
dc.identifier.urihttps://doi.org/10.3390/appliedchem3020021
dc.publisherMDPI
dc.relationThis work was supported by the Estonian Research Council grant (PRG1441).eng
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)eng
dc.rights© 2023 by the authors.eng
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/eng
dc.subjectnuclear magnetic resonanceeng
dc.subjectfast protein liquid chromatographyeng
dc.subjectfish freshnesseng
dc.subjectdietary nucleotideseng
dc.subjectnucleotide salvageeng
dc.subjectarticleseng
dc.titleManifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectraeng
dc.typeinfo:eu-repo/semantics/articleeng

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