Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra
Laen...
Kuupäev
2023
Kättesaadav alates
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
MDPI
Abstrakt
Dietary nucleotides and nucleosides, primarily inosine monophosphate (IMP) and the
adenine nucleotide pool (ANP), are widely considered as essential nutrients responsible for multiple
biological functions. Food prepared from meat and fish is the main source of these substances in
the human diet, and it is extremely important to implement storage and processing techniques
ensuring their maximum preservation and even accumulation during maturation or conditioning.
In experiments with freshly refrigerated grass carp and defrosted Alaska pollock fillets it was
discovered, initially using Fast Protein and Metabolites Liquid Chromatography and the ATP-
bioluminescence test, and afterwards validated by NMR spectroscopy, that heat treatment identical
to conventional culinary processing in aqueous or wet media at temperatures above 62 ◦C leads
to nucleotide salvage (recovery) in aged fish. A significant increase in the concentration of IMP,
and even an emergence of ANP substances, were reliably demonstrated in fish samples which had
already partially or fully lost these components during prolonged storage due to the ATP breakdown
metabolic reactions. Owing to this recovery, the nutritive value of ready-to-eat food can be higher
than was initially evaluated in raw products before heat treatment: an effect that should certainly be
considered in practical nutrition. Moreover, it is necessary to reconsider the widely acknowledged
system of indices of freshness based on nucleotides and nucleosides elaborated a long time ago for
raw meat and fish products.
This research was partially financially supported by the Estonian Research Council grant (PRG 1441) of the Estonian University of Life Sciences (A.S., P.R., T.P., M.R.).
This research was partially financially supported by the Estonian Research Council grant (PRG 1441) of the Estonian University of Life Sciences (A.S., P.R., T.P., M.R.).
Kirjeldus
Märksõnad
nuclear magnetic resonance, fast protein liquid chromatography, fish freshness, dietary nucleotides, nucleotide salvage, articles
Viide
Sünter, A.; Kuznetsov, A.; Raudsepp, P.; Püssa, T.; Toom, L.; Konoplev, G.; Stepanova, O.S.; Stepanova, O.V.; Lyalin, D.; Frorip, A.; et al. Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra. AppliedChem 2023, 3, 334–349. https://doi.org/10.3390/ appliedchem3020021