Isolation of streptomycetes causing common scab from 3-years old potato samples from South America
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Kuupäev
2020
Kättesaadavus
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Abstrakt
In this paper, we aimed at assessing the best conditions for the isolation of
actinobacteria from old potato samples. A set of media and pretreatments were tested. The
optimal were chosen for the isolation of actinobacteria from potatoes from Peru, Chile and
Argentina. Isolates were tested on the presence of thaxtomin phytotoxin by amplification of the
respective gene. Phylogenetic position of strains was compared with their geographical origin,
pathogenic potential and existence of common scab (CS) symptoms on potato sample. We
demonstrated that RNAlater can be successfully used for the long-term preservation of potato
peel for subsequent isolation of actinobacteria on R2A medium. Many streptomycetes were
thaxtomin-positive, though they are distantly-related to described pathogens causing CS. Genus
Nocardia was first reported to be thaxtomin-positive. Potentially pathogenic strains were isolated
not only from infected potato but also from those lacking CS symptoms. Some strains from
scabby potatoes were thaxtomin-negative.
Kirjeldus
Märksõnad
common scab, streptomycetes, thaxtomin, potato disease, articles