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Metabolite profiling, terpenoid and kaurenoic acid production of Adenostemma platyphyllum at different concentrations of hydroponic solutions in the wick system

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Kuupäev

2024

Kättesaadav alates

Autorid

Tamsin, A.H.N.
Nurfalah, R.
Trivadila
Batubara, I.
Rafi, M.
Ridwan, T.
Aziz, S.A.
Takemori, H.

Ajakirja pealkiri

Ajakirja ISSN

Köite pealkiri

Kirjastaja

Estonian University of Life Sciences

Abstrakt

Adenostemma platyphyllum, a medicinal plant belonging to the Asteraceae family, has gained increasing attention due to its potential as a source of bioactive compounds with diverse therapeutic properties but has not been widely cultivated. This work aims to obtain the optimum concentration of AB-mix solution to produce higher terpenoid and kaurenoic acid, as well as metabolite profiling in cultivating A. platyphyllum using a hydroponic wick system. This research uses a one-factor randomized block design of different concentrations of AB-mix nutrient solutions. Total terpenoids were quantified using the UV-Vis spectrophotometric method, total kaurenoic acid was determined using a high-performance liquid chromatography (HPLC) method, and the metabolite profiling was analyzed using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) instrument. Several terpenoid compounds have been identified in A. platyphyllum, including Ent-17-Oxo-15-kauren-19-oic acid, andrographolide, cafestol, alphaFarnesene, curcumene, as well as ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic acid (11αOH-KA) and 11α,15-dihydroxy-16-kauren-19-oic acid (11α,15OH-KA), which belong to the kaurenoic acid group. The plants had the highest total terpenoid and kaurenoic acid found in 1,300 mg L-1 nutrient concentrations. On the other hand, the highest terpenoid and kaurenoic acid productivity were found in plants with 900 and 1,300 mg L-1 AB-mix solution, respectively. Therefore, the optimum concentration of nutrient solution to produce optimum terpenoid and kaurenoic acid levels in A. platyphyllum cultivation by hydroponic wick system was 1,300 mg L-1.

Kirjeldus

Received: March 9th, 2024 ; Accepted: June 27th, 2024 ; Published: August 29th, 2024 ; Correspondence: ime@apps.ipb.ac.id

Märksõnad

Adenostemma platyphyllum, hydroponic, kaurenoic acid, plant growth, total terpenoid, articles

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