Koerte akuutse faasi proteiinid põletiku markerina: tervete ja püometra diagnoosiga koerte võrdlus
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Kuupäev
2013
Kättesaadav alates
ainult raamatukogus, only in library
Autorid
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Abstrakt
Lõputöö jaoks koguti andmed Eesti Maaülikooli väikeloomakliinikus (EMÜ VLK) ning määrati valitud populatsioonil akuutse faasi proteiine (APP). Uuringu eesmärgiks oli välja selgitada APP-de kvantitatiivsed muutused püometra diagnoosiga koertel ning võrrelda saadud tulemusi kontrollrühma ehk tervete koertega.
Töös uuriti kahte koerte rühma. Esimese rühma ehk kontrollrühma moodustasid väikeloomakliiniku kliiniliselt terved patsiendid (kokku 53 koera). Rühmas olid nii emased ja isased kui ka kastreeritud ja steriliseeritud koerad. Teise koerterühma moodustasid steriliseerimata emased koerad, kellel oli diagnoositud püometra (kokku 35 koera). Kõikidel koertel varieerusid nii vanus, kehakaal, kehakonditsioon kui ka tõug.
EMÜ VLK laboratooriumis määrati uuringus planeeritud biokeemilised vereparameetrid ja Eesti Maaülikooli hematoloogia ja biokeemia laboris tehti hematoloogiline vereuuring. Seerumi amüloid A (SAA), C-reaktiivne proteiin (CRP) ja haptoglobiin (Hp) analüüsiti EMÜ mükobakteriooside laboratooriumis, kasutades SAA ja CRP määramiseks kommertsiaalset ensüümset immunosorptsioonianalüüsi (ELISA) ja Hp määramiseks kommertsiaalset kolorimeetrilist testi vastavalt juhenditele.
Püometra diagnoosiga koertel täheldati CRP puhul 67, SAA puhul 195, Hp puhul aga 6,4 korda suuremat plasma kontsentratsiooni kui kontrollrühma ehk tervetel koertel. Samuti leiti optimaalsed piirväärtused CRP (> 6,6 mg/l), SAA (> 41,1 mg/l), Hp (> 3,8 g/l), albumiini (≤ 31 g/l) ja leukotsüüdide (WBC) arvu (17 × 109 l) jaoks, kasutades ROC-analüüsi (receiver operating characteristic). Kontrollrühma koerte populatsioonil määrati APP-de ja WBC arvu referentsväärtused, mis ei erinenud oluliselt optimaalsetest piirväärtustest. Võrreldes käesolevas uuringus tervetel koertel (kontrollrühma koertel) saadud APP-de kontsentratsioone teiste varem tehtud uuringute tulemustega, ei täheldatud märkimisväärseid erinevusi (v.a SAA).
The material used in this paper was collected from dogs in the Small Animal Clinic of the Estonian University of Life Sciences where acute phase proteins and white blood cell count was measured. The purpose of the study was to determine APP-s quantitative changes in dogs with pyometra and compare results to the healthy dogs. This paper studied dogs in two groups. First group or control group was formed from the clinic’s clinically healthy patients (53 dogs). The group included male and female dogs as well as neutered and sterilised animals. Second group was formed by the dogs that were diagnosed with pyometra (35 dogs). All dogs were of different age, weight, body condition index and breed. In the clinic’s laboratory biochemical blood parameters were measured, the complete blood count was analyzed in the haematology and biochemistry laboratory of the Estonian University of Life Sciences. Serum amyloid A (SAA), C-reactive protein (CRP) and haptoglobin (Hp) were analysed in the laboratory of the mycobacterioses of the Estonian University of Life Science using commercial enzyme-linked immunosorbent assays (ELISA) and for Hp measuring the commercial colorimetric assay was used according to the instructions. In case of CRP testing, it showed that plasma concentration was 67 times, in case of SAA testing was 195 times, in case of Hp testing 6,4 times higher in the dogs with pyometra compared to the healthy control group dogs. We also defined cut-off values for CRP (> 6,6 mg/l), SAA (> 41,1 mg/l), Hp (> 3,8 g/l), albumin (≤ 31 g/l) and WBC counts (17 ×109 l) using ROC-analysis (receiver operating characteristic). During this study we also defined APPs and WBC counts referential intervals for the control group dogs, which were not significantly different from cut-off values. APP-s concentration levels in healthy dogs in this study were compared to the other existing studies and no significant differences were observed (except for SAA).
The material used in this paper was collected from dogs in the Small Animal Clinic of the Estonian University of Life Sciences where acute phase proteins and white blood cell count was measured. The purpose of the study was to determine APP-s quantitative changes in dogs with pyometra and compare results to the healthy dogs. This paper studied dogs in two groups. First group or control group was formed from the clinic’s clinically healthy patients (53 dogs). The group included male and female dogs as well as neutered and sterilised animals. Second group was formed by the dogs that were diagnosed with pyometra (35 dogs). All dogs were of different age, weight, body condition index and breed. In the clinic’s laboratory biochemical blood parameters were measured, the complete blood count was analyzed in the haematology and biochemistry laboratory of the Estonian University of Life Sciences. Serum amyloid A (SAA), C-reactive protein (CRP) and haptoglobin (Hp) were analysed in the laboratory of the mycobacterioses of the Estonian University of Life Science using commercial enzyme-linked immunosorbent assays (ELISA) and for Hp measuring the commercial colorimetric assay was used according to the instructions. In case of CRP testing, it showed that plasma concentration was 67 times, in case of SAA testing was 195 times, in case of Hp testing 6,4 times higher in the dogs with pyometra compared to the healthy control group dogs. We also defined cut-off values for CRP (> 6,6 mg/l), SAA (> 41,1 mg/l), Hp (> 3,8 g/l), albumin (≤ 31 g/l) and WBC counts (17 ×109 l) using ROC-analysis (receiver operating characteristic). During this study we also defined APPs and WBC counts referential intervals for the control group dogs, which were not significantly different from cut-off values. APP-s concentration levels in healthy dogs in this study were compared to the other existing studies and no significant differences were observed (except for SAA).
Kirjeldus
Märksõnad
CRP, SAA, pH, püometra, põletik, koerte haigused, magistritööd