The use of DNA markers for the evaluation of maize lines and hybrids based on cytoplasmic male sterility
Laen...
Kuupäev
2020
Kättesaadavus
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Abstrakt
The use of cytoplasmic male sterility (CMS) is very important for the production of
maize hybrids. The new inbred lines and hybrids of maize of Ukrainian breeding were studied.
In the field, four pairs of sterile analogue of line RL106S were obtained during four backcrossing
(17М, 19М, 23М, 27М and 29М) and maintainer lines of RL106fS (18М, 20М, 24М, 28М and
30М) for S type cytoplasm and RL108C (195С, 201С, 205С, 207С, 209С) і RL108fC (196С,
202С, 206С, 208С, 210С) for C type respectively. For S type, the following combinations were
obtained: RL23S×RL106fS, RL107S×RL106fS, RL98S×RL106fS, RL105S×RL106fS,
RL113S×RL106fS and for C type: RL109C×RL108fC, RL110C×RL108fC, RL112C×RL108fC,
RL114C×RL108fC, RL115C×RL108fC. The obtained hybrid combinations were planted the
following year in a control nursery for field trials. According to the results of the field assessment,
all the hybrids were sterile. The types of sterility of the studied lines and hybrids were determined
using polymerase chain reaction (PCR) with specific primers for C and S types of cytoplasms.
The presence of specific amplicons 398 and 799 bp was determined in sterile lines with C and S
types of cytoplasm, respectively. Amplicons 398 and 799 bp were identified in simple-cross and
simple reconstituted hybrids on a sterile basis, and can be used to determine the type of hybrid
and its maternal component at the stages of selection and examination of new hybrids.
Kirjeldus
Märksõnad
Zea mays L., DNA markers, cytoplasmic sterility, articles