Harnessing Nature’s Defence: The Antimicrobial Efficacy of Pasteurised Cattle Milk-Derived Extracellular Vesicles on Staphylococcus aureus ATCC 25923
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Kuupäev
2024
Kättesaadavus
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
MDPI
Abstrakt
Increasing antimicrobial resistance (AMR) challenges conventional antibiotics, prompting
the search for alternatives. Extracellular vesicles (EVs) from pasteurised cattle milk offer promise,
due to their unique properties. This study investigates their efficacy against five pathogenic bacteria,
including Staphylococcus aureus ATCC 25923, aiming to combat AMR and to develop new therapies.
EVs were characterised and tested using various methods. Co-culture experiments with S. aureus
showed significant growth inhibition, with colony-forming units decreasing from 2.4 × 105 CFU/mL
(single dose) to 7.4 × 104 CFU/mL (triple doses) after 12 h. Milk EVs extended lag time (6 to 9 h)
and increased generation time (2.8 to 4.8 h) dose-dependently, compared to controls. In conclusion,
milk EVs exhibit dose-dependent inhibition against S. aureus, prolonging lag and generation times.
Despite limitations, this suggests their potential in addressing AMR.
Kirjeldus
Märksõnad
Staphylococcus aureus, extracellular vesicles, novel antimicrobials, pasteurised milk, antibacterial activity, dietary EVs, articles
Viide
Sapugahawatte, D.N.; Godakumara, K.; Mäesaar, M.; Ekanayake, G.; Midekessa, G.B.; Prasadani, M.; Kodithuwakku, S.; Roasto, M.; Andronowska, A.; Fazeli, A. Harnessing Nature’s Defence: The Antimicrobial Efficacy of Pasteurised Cattle Milk-Derived Extracellular Vesicles on Staphylococcus aureus ATCC 25923. Int. J. Mol. Sci. 2024, 25, 4759. https://doi.org/10.3390/ ijms25094759