Development of an efficient regeneration system for bombarded calli from immature embryos of Moroccan durum wheat varieties
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Kuupäev
2019
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Abstrakt
One of the biggest obstacles limiting genetic transformation of durum wheat is the lack
of an efficient regeneration system for bombarded tissues. Our study aims to optimize culture
conditions for regenerating bombarded calli from immature embryos of four durum wheat
varieties ‘Amria’, ‘Chaoui’, ‘Isly’ and ‘Marouane’, through comparing the effects of
phytohormones (IAA, zeatin and their interaction) and nitrogen amount and sources on callus
induction and plant regeneration. Both tested induction media induced approximately the same
rate of induced calli for all the tested varieties. However, the interaction of the induction and the
regeneration media showed a highly significant effect on plantlet regeneration for all tested
varieties. After bombardment, IM1/RM2 combination proved to be the favourable medium with
up to 200% and 120% plantlets regenerated for ‘Chaoui’ and ‘Isly’ varieties respectively.
Encouraging results obtained in this study will help to promote the research in genetic
transformation and its improvement.
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Märksõnad
bombarded calli, callus induction, durum wheat, immature embryos, plantlets regeneration, articles