Bovine colostrum whey: Postpartum changes of particle size distribution and immunoglobulin G concentration at different filtration pore sizes
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Kuupäev
2020
Kättesaadav alates
Autorid
Sats, A.
Kaart, T.
Poikalainen, V.
Aare, A.
Lepasalu, L.
Andreson, H.
Jõudu, I.
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Abstrakt
Bovine colostrum, as vital as it is for calves, is also
a valuable source of functional components with rich
health benefits for humans. Bovine colostrum whey
consists of a large number of bioactive proteins and
peptides. The most abundant of these is IgG. Particle
size distribution (PSD) is an important feature of many
of the processes in the dairy food industries. Despite
this, scientific literature on PSD of colostrum whey is
scarce. The goal of this research was to describe bovine
colostrum whey PSD with an emphasis on postpartum
milking time, filtration (pore size 450, 100, and 20
nm), IgG concentration, and lactation number. For this
purpose, 4 postpartum milking colostrum samples were
sequentially milked from 46 Holstein cows at 12 ± 1
h intervals. Colostrum whey was prepared by renneting
and diluted (1:200) for PSD analyses by a Malvern
Zetasizer Nano ZS (Malvern Instruments Ltd., Malvern,
UK). Immunoglobulin G concentration of these diluted
colostrum whey samples were analyzed by an Octet
K2 (Molecular Devices LLC, San Jose, CA) system.
Linear mixed model analysis revealed significant effects
of filter pore size, postpartum milking, and lactation
on colostrum whey IgG concentrations. The percentage
of particles in the size interval 5 to 15 nm (the
hydrodynamic diameter of IgG is around 10 nm) had
an intermediate positive correlation (r = 0.50) with IgG
concentration. Furthermore, we showed that PSD was
associated with IgG concentration, postpartum milking
time, and lactation number. The PSD measurement
results showed the mean hydrodynamic diameter of 100
nm pore size filtered colostrum whey to be around 10
nm. This, with the IgG concentration results, suggests
that even though the size of IgG is around 10 nm, a
100 nm pore size is adequate for membrane-involved
IgG separations. In terms of energy efficiency of the filtration process, the use of a larger filter pore size
can make a remarkable difference, for example, in
pressurizing and cooling costs. Our work contributes
to the development of sustainable and widely available
colostrum-derived food and feed supplements.
Kirjeldus
Märksõnad
bovine colostrum whey, particle size distribution, immunoglobulin G, filtration pore size, articles