Antibiotic Resistance in Campylobacter spp. Isolated from Broiler Chicken Meat and Human Patients in Estonia
Laen...
Kuupäev
2022
Kättesaadav alates
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
MDPI
Abstrakt
Poultry meat is considered the most important source of Campylobacter spp. Because of ris-
ing antimicrobial resistance in Campylobacter spp., this study investigated the antimicrobial resistance
of Campylobacter isolates from fresh broiler chicken meat originating from the Baltic countries sold in
Estonian retail settings. Additionally, human clinical isolates obtained from patients with Campylobacter
enteritis in Estonia were analysed. The aim of this study was to investigate the susceptibility of
Campylobacter spp. to nalidixic acid, ciprofloxacin, tetracycline, streptomycin, erythromycin and gen-
tamicin. The broth microdilution method with the EUCAMP2 panel was used for MIC determination
and antimicrobial mechanisms were analysed using WGS data. A total of 46 Campylobacter strains
were analysed, of which 26 (42.6%) originated from Lithuanian, 16 (26.2%) from Latvian, and 4 (6.6%)
from Estonian fresh broiler chicken meat. In addition, 15 (24.6%) Campylobacter strains of patients
with campylobacteriosis were tested. The antimicrobial resistance patterns of Campylobacter spp.
isolated from fresh broiler chicken meat samples of Estonian, Latvian and Lithuanian origin collected
in Estonian retail, and from patients with Campylobacter enteric infections, were determined. A total
of 46 (75%) of the isolates tested were C. jejuni and 15 (25%) were C. coli. Campylobacter resistance
was highest to nalidixic acid (90.2% of strains) and ciprofloxacin (90.2%), followed by tetracycline
(57.4%), streptomycin (42.6%) and erythromycin (6.6%). All strains were sensitive to gentamicin.
Additionally, antimicrobial resistance genes and point mutations were detected in 27 C. jejuni and 8 C.
coli isolates previously assigned as resistant with the phenotypic method. A high antibiotic resistance
of Campylobacter spp. in Lithuanian- and Latvian-origin broiler chicken meat and Estonian clinical
isolates was found. Similar antibiotic resistance patterns were found for broiler chicken meat and
human Campylobacter isolates.
This work was supported by the Estonian Research Council grant PRG1441. WORLDCOM project of the One Health European Joint Programme (OHEJP) consortium and received funding from the European Union’s Horizon 2020 Research and Innovation programme [grant number 773830].
This work was supported by the Estonian Research Council grant PRG1441. WORLDCOM project of the One Health European Joint Programme (OHEJP) consortium and received funding from the European Union’s Horizon 2020 Research and Innovation programme [grant number 773830].
Kirjeldus
Märksõnad
Campylobacter spp, antibiotic resistance, multidrug resistance, fresh broiler chicken meat, resistance genes, articles
Viide
Citation: Tedersoo, T.; Roasto, M.; Mäesaar, M.; Häkkinen, L.; Kisand, V.; Ivanova, M.; Valli, M.H.; Meremäe, K. Antibiotic Resistance in Campylobacter spp. Isolated from Broiler Chicken Meat and Human Patients in Estonia. Microorganisms 2022, 10, 1067. https://doi.org/ 10.3390/microorganisms10051067