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Improved protocols for testing agrochemicals in bees: Deliverable D3.2




Kättesaadav alates


Medrzycki, Piotr
Hellström, Sara
Straw, Edward
Linguadoca, Alberto
Jürison, Margret
Alaux, Cedric
Barascou, Lena
Brown, Mark
Costa, Cecilia
De la Rúa, Pilar

Ajakirja pealkiri

Ajakirja ISSN

Köite pealkiri




Within the PoshBee Project, we have worked out precise protocols for testing the toxicological endpoints and metabolisation rate of agrochemicals on and in bees. Three agrochemical classes (insecticides, fungicides and herbicides) were tested on three bee groups (honey bees, bumble bees and solitary mason bees). The specific protocols, different for each combination of the pesticide class and bee group, were designed based on the existing toxicity testing methods and modified according to the specificities of the different bee groups, their castes and the different exotoxicological features of the compounds. Sulfoxaflor, Azoxystrobin, and Glyphosate were used respectively as examples of the three pesticide classes. Apis mellifera, Bombus terrestris and Osmia bicornis were chosen as the examples of the three bee groups. The protocols for honey bees and bumble bees were designed based on the official existing guidelines edited by OECD. Nevertheless, given that the OECD guidelines provide recommendations to fit with most agrochemicals, many of the parameters are defined with low precision in order to be flexible and adapt to different categories of molecules. While OECD has no guidelines for testing solitary bees, we used information from the unpublished ring test protocols for Osmia bicornis by the ICPPR non- Apis working group.
Prepared under contract from the European Commission; Grant agreement No. 773921; EU Horizon 2020 Research and Innovation action.



honey bees, bumblebees, agrochemicals, reports


Medrzyck, P., Hellström, S., Straw,E., Linguadoca, A., Jürison, M., Alaux, C. et al. (2021). Improved protocols for testing agrochemicals in bees. Deliverable D3.2. EU Horizon 2020 PoshBee Project, Grant agreement No. 773921