Serum Amyloid A Changes in Mares Following Ovum Pick-Up
| dc.contributor.advisor | Reilent, Andres (advisor) | |
| dc.contributor.advisor | Kavak, Ants (advisor) | |
| dc.contributor.author | Autio, Merja Helena | |
| dc.contributor.department | Chair of Veterinary Biomedicine and Food Hygiene | eng |
| dc.date.accessioned | 2025-06-03T10:21:00Z | |
| dc.date.available | 2025-06-03T10:21:00Z | |
| dc.date.defensed | 2025-06-04 | |
| dc.date.issued | 2025 | |
| dc.description | Final Thesis Curriculum in Veterinary Medicine | eng |
| dc.description.abstract | Serum amyloid A is a sensitive acute-phase protein in horses that is useful for detecting inflammation and tissue injuries. In assisted reproductive technologies, particularly ovum pick-up, understanding the inflammatory response is important for evaluating the procedural safety and effects on animal welfare. While ovum pick-up is minimally invasive, its potential to induce systemic inflammation remains understudied in horses. This study analysed serum amyloid A in mares following an ovum pick-up procedure to determine the inflammatory response to the procedure. Serum amyloid A was measured from 10 mares before (0 h) and after ovum pick-up procedure (24 h, 48 h, and 72 h). Altogether 19 ovum pick-ups were conducted. Median serum amyloid A levels increased minimally, reaching a peak at 24 h (0,58 mg/L) with a maximum 2,85-fold rise from baseline (0,20 mg/L), before declining to near baseline levels by 72 h (0,31 mg/L). No significant correlation was found between the serum amyloid A levels and mare age, haemolysis or repeated ovum pick-up procedures. The observed serum amyloid A levels were within the range of healthy horses, as described in previous studies. These findings suggest that ovum pick-up induces minimal and transient systemic reaction in horses, which supports its safety as procedure. However, the small sample size (10 mares, 19 procedures) limits generalisation and further research is needed. | eng |
| dc.description.abstract | Seerumi amüloid A (SAA) on hobustel tundlik ägeda faasi valk, mida kasutatakse põletiku ja koekahjustuste tuvastamiseks. Abistavates reproduktiivtehnoloogiates, eriti transvaginaalsel foliikulipunktsioonil (OPU), on põletikulise vastuse mõistmine oluline protseduuri ohutuse ja loomade heaolu mõjude hindamiseks. Kuigi OPU on minimaalselt invasiivne, on selle võimalik süsteemne põletikuline mõju hobustel väheuuritud. Käesolev uuring analüüsis märade seerumi amüloid A taset pärast OPU protseduuri, et hinnata protseduuri põletikulist vastust. Seerumi amüloid A taset mõõdeti 10 märal enne (0 h) ja pärast OPU protseduuri (24 h, 48 h ja 72 h). Kokku viidi läbi 19 OPU protseduuri. Mediaanne seerumi amüloid A tase tõusis minimaalselt, saavutades tipu 24 h jooksul (0,58 mg/L), mis oli maksimaalselt 2,85 korda kõrgem baastasemest (0,20 mg/L), ning langes 72 h jooksul peaaegu baastasemele tagasi (0,31 mg/L). Seerumi amüloid A taseme ja märavanuse, hemolüüsi või korduvate OPU protseduuride vahel ei leitud olulist seost. Vaadeldud seerumi amüloid A tasemed jäid tervete hobuste normivahemikku, nagu on kirjeldatud varasemates uuringutes. Need leiud viitavad, et OPU põhjustab hobustel minimaalset ja mööduvat süsteemset reaktsiooni, mis kinnitab selle protseduuri ohutust. Siiski väike valim (10 mära, 19 protseduuri) piirab tulemuste üldistatavust ning on vaja edasisi uuringuid. | est |
| dc.identifier.uri | http://hdl.handle.net/10492/9937 | |
| dc.publisher | Eesti Maaülikool | |
| dc.rights | info:eu-repo/semantics/openAccess | eng |
| dc.rights | Attribution 4.0 International (CC BY NC ND 4.0) | eng |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | magistritööd | est |
| dc.subject | Ovum pick-up | eng |
| dc.subject | serum amyloid A | eng |
| dc.subject | assisted reproduction | eng |
| dc.subject | equine health | eng |
| dc.title | Serum Amyloid A Changes in Mares Following Ovum Pick-Up | eng |
| dc.title.alternative | Transvaginaalse Folliikuulipunktsiooni Järgne Seerumi Amüloid A muutus märadel | est |
| dc.type | Master Thesis | eng |
| rioxxterms.freetoread.startdate | 04.09.2025 |