Stimulation and production of some active compounds in the Chia plant with melatonin in vitro
Laen...
Kuupäev
2022
Kättesaadav alates
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Estonian University of Life Sciences
Abstrakt
A laboratory experiment was carried out in the tissue culture laboratories of the College
of Agriculture \ University of Anbar \ Iraq from 1/2/ 2021 till 1/7/2021, to multiply and produce
some active compounds in Chia in vitro. Nutrition medium (MS) (Murashige and Skoog) was
used to propagate chia plants. The culture medium was enriched with benzyl adenine regulator at
MS concentrations, 1, 2, and 3 mg L-1
with five replicates for each one, respectively, in order in
the multiplication phase, to know its effect on some morphological characteristics of Chia plants
such as branch length, nodes and leaflets number. Also, brassinolide was laid out of 0, 1 and
2 mg L-1
, while NAA added of 0, 1, 2 and 3 mg L-1
with five replicates for each one respectively,
were added to the culture media at the rooting stage, in order to test its effectiveness in improving
the formation of rootlets of Chia plants. In another experiment, the culture medium was enriched
with different concentrations of the melatonin PGR 0, 1, 2, and 3 mg L-1
, with five replicates for
each one, respectively, in order to study the effect of melatonin in the production of active
compounds. The results showed that enrichment of the culture medium with benzyl adenine at a
concentration of 1 mg L-1 increased the length, branches number and nodules number in plantlets
(9.46 cm branch-1
, 5.45 branch plantlet-1
, and 9.57 nodes shoot-1
). The combined effect of
Brassinolide 1 mg L-1 and 3 mg L-1 NAA improved both number of rootlets and their length. of
10.5 rootlets plantlet-1 and 5.7 cm, respectively. Also, the addition of melatonin to the culture
medium significantly diversifying active compounds in term of quantity and quality
characteristics. The concentration of 1 mg L-1 of melatonin gave the highest number of active
compounds (25 compounds), however the etheric compounds and fatty acid derivatives were the
highest. The percentage of etheric compounds was 80.0% by adding 3 mg L-1 of melatonin, and
the percentage of fatty acid derivatives reached 16.36% of the total area ratio by adding 1 mg L-1.
Kirjeldus
Received: August 1st, 2022 ; Accepted: October 17th, 2022 ; Published: October 24th, 2022 ; Correspondence: ds.dr.ali.fadaam@uoanbar.edu.iq
Märksõnad
chia, melatonin, in vitro, active compounds, articles