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Optimisation of biologically active compounds ultrasound assisted extraction from potatoes using response surface methodology

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dc.contributor.authorZarins, R.
dc.contributor.authorKruma, Z.
dc.contributor.authorSkrabule, I.
dc.date.accessioned2018-05-09T10:08:54Z
dc.date.available2018-05-09T10:08:54Z
dc.date.issued2018
dc.descriptionArticleeng
dc.description.abstractPotato ( Solanum tuberosum L.) is source of p henolic compounds and from plant matrixes can be extracted by several methods. In recent years ultrasound assisted extraction has become more popular due to its efficiency for recovery of phenolic compounds and antioxidants and response surface methodology is an effective tool for optimisation of extraction procedure by evaluating different variables and their interaction. The aim of the current research was to optimize ultrasound assisted extraction of biologically active compounds from potatoes by respons e surface methodology. For experiment purple - flesh potato variety ‘Blue Congo’ was selected. Control sample was extracted by stirring for 1 hour. Box - Behnken design was used for optimization of extraction conditions from fresh potatoes and as variables wer e selected: ethanol concentration (% v/v), hydrochloric acid concentration (molarity) and time (min). For extracts as responses total phenolic, total flavonoid, total anthocyanin content and antioxidant activity (DPPH, ABTS+ scavenging activity) were deter mined using a spectrophotometric methods. Significant models were obtained for antocyanins, total phenols and DPPH radical scavenging activity. Optimisation of extraction showed that for maximising all responses optimal HCl concentration is 2.5M, ethanol c oncentration 79.4% and extraction time 60 minutes, resulting in following responses: 57.41 mg 100 g - 1 of anthocyanins, 238.52 mg 100 g - 1 of TPC, 24.58 mM TE 100 g - 1 of DPPH scavenging activity and 12.99 mM TE 100 g - 1 of ABTS scavenging activity. Convention al extraction method showed significantly lower results. It could be concluded that ultrasound assisted extraction is effective method for recovery of phenolic compounds and solvents and extraction time is significant parameter influencing efficiency.eng
dc.identifier.issn1406-894X
dc.identifier.publicationAgronomy Research, 2018, vol. 16, Special Issue 2, pp. 1534-1545eng
dc.identifier.urihttp://hdl.handle.net/10492/3942
dc.identifier.urihttp://dx.doi.org/10.15159/ar.18.116
dc.rights.holderCopyright 2009 by Estonian University of Life Sciences, Latvia University of Agriculture, Aleksandras Stulginskis University, Lithuanian Research Centre for Agriculture and Forestry. No part of this publication may be reproduced or transmitted in any form, or by any means, electronic or mechanical, incl. photocopying, electronic recording, or otherwise without the prior written permission from the Estonian University of Life Sciences, Latvia University of Agriculture, Aleksandras Stulginskis University, Lithuanian Research Centre for Agriculture and Forestryeng
dc.subjectpurple-flesh potatoeseng
dc.subjectphenolicseng
dc.subjectultrasound assisted extractioneng
dc.subjectresponse surface methodologyeng
dc.subjectBox-Behnken designeng
dc.subjectarticleseng
dc.titleOptimisation of biologically active compounds ultrasound assisted extraction from potatoes using response surface methodologyeng
dc.typeArticleeng

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2018, Vol. 16, Special Issue 2