Optimisation of biologically active compounds ultrasound assisted extraction from potatoes using response surface methodology
Laen...
Kuupäev
2018
Kättesaadav alates
Autorid
Zarins, R.
Kruma, Z.
Skrabule, I.
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Abstrakt
Potato (
Solanum tuberosum
L.) is source of p
henolic compounds and from plant
matrixes can be extracted by several methods. In recent years ultrasound assisted extraction has
become more popular due to its efficiency for recovery of phenolic compounds and antioxidants
and response surface methodology
is an effective tool for optimisation of extraction procedure
by evaluating different variables and their interaction. The aim of the current research was to
optimize ultrasound assisted extraction of biologically active compounds from potatoes by
respons
e surface methodology. For experiment purple
-
flesh potato variety ‘Blue Congo’ was
selected. Control sample was extracted by stirring for 1 hour. Box
-
Behnken design was used for
optimization of extraction conditions from fresh potatoes and as variables wer
e selected: ethanol
concentration (% v/v), hydrochloric acid concentration (molarity) and time (min). For extracts as
responses total phenolic, total flavonoid, total anthocyanin content and antioxidant activity
(DPPH, ABTS+ scavenging activity) were deter
mined using a spectrophotometric methods.
Significant models were obtained for antocyanins, total phenols and DPPH radical scavenging
activity. Optimisation of extraction showed that for maximising all responses optimal HCl
concentration is 2.5M, ethanol c
oncentration 79.4% and extraction time 60
minutes, resulting in
following responses: 57.41 mg 100 g
-
1
of anthocyanins, 238.52 mg 100
g
-
1
of TPC, 24.58
mM
TE
100
g
-
1
of DPPH scavenging activity and 12.99 mM TE
100
g
-
1
of ABTS scavenging activity.
Convention
al extraction method showed significantly lower results. It could be concluded that
ultrasound assisted extraction is effective method for recovery of phenolic compounds and
solvents and extraction time is significant parameter influencing efficiency.
Kirjeldus
Article
Märksõnad
purple-flesh potatoes, phenolics, ultrasound assisted extraction, response surface methodology, Box-Behnken design, articles