Antimicrobial sensitivity of campylobacter spp. isolated from fresh broiler chicken meat of Estonian retail level
| dc.contributor.advisor | Roasto, Mati | |
| dc.contributor.advisor | Meremäe, Kadrin | |
| dc.contributor.author | Valli, Marikki Heidi | |
| dc.date.accessioned | 2022-05-27T12:32:04Z | |
| dc.date.available | 2022-05-27T12:32:04Z | |
| dc.date.defensed | 2022-06-10 | |
| dc.date.issued | 2022 | |
| dc.description | Final Thesis Curriculum in Veterinary Medicine | eng |
| dc.description.abstract | Campylobacter spp. are causing most of the human bacterial enteric infections in European Union, and broiler chicken meat is the most important source of Campylobacter jejuni. Present study aimed to determine the antimicrobial resistance of Campylobacter spp. isolated from fresh broiler chicken meat of Estonian, Latvian and Lithuanian origin purchased at Estonian retail level, and among human Campylobacter isolates. Minimal inhibitory concentrations for tested antimicrobials were determined by using the EUCAMP2 method. Campylobacter isolates were tested against erythromycin, ciprofloxacin, tetracycline, streptomycin, gentamicin and nalidixic acid. Very high resistance (100%) to fluoroquinolones was determined for Lithuanian and Latvian origin Campylobacter isolates originated from fresh broiler chicken meat at Estonian retail level. Among clinical (human) isolates the resistance to fluoroquinolones was 86.7%. High resistance to tetracycline was determined for Lithuanian origin broiler chicken meat and Estonian human isolates, respectively 76.9% and 80.0%. The proportion of streptomycin resistant strains was high for Latvian and Lithuanian origin broiler chicken meat Campylobacter isolates, respectively 68.8% and 42.3%. The overall proportion of multidrug resistance among studied Campylobacter isolates was 26.2%. All isolates were sensitive to gentamicin. Low resistance to erythromycin was found. All studied Estonian broiler chicken meat isolates were sensitive to all studied antimicrobials. High resistance among Lithuanian and Latvian origin broiler chicken meat Campylobacter isolates, and among clinical isolates is public health concern. To reduce antimicrobial resistance, the control of the use of antibiotics at broiler chicken meat farm level should be more effective, and antibiotics cannot be used in preventative and metaphylactic treatments, also in growth promotion purposes. | eng |
| dc.description.abstract | Termofiilsed kampülobakterid põhjustavad Euroopa Liidus enim bakteriaalseid sooleinfektsioone inimestel ning kanabroileriliha on Campylobacter jejuni kõige olulisem kampülobakterioosi allikas. Käesoleva uuringu eesmärk oli määrata Eesti jaemüügi tasandil ostetud värskest Eesti, Läti ja Leedu päritolu kanabroilerilihast ning Eesti inimestelt isoleeritud Campylobacter spp. antimikroobne resistentsus. Minimaalsed inhibeerivad kontsentratsioonid määrati kasutades EUCAMP2 meetodit. Kampülobakterite isolaate testiti erütromütsiini, tsiprofloksatsiini, tetratsükliini, streptomütsiini, gentamütsiini ja nalidiksiinhappe suhtes. Väga kõrge (100%) resistentsus fluorokinoloonidele määrati Eesti jaemüügi tasandil Leedu ja Läti päritolu Campylobacter isolaatidel, mis pärinesid värskest broilerilihast. Kliiniliste (inimese) isolaatide hulgas oli resistentsus fluorokinoloonide suhtes 86,7%. Kõrge resistentsus tetratsükliini suhtes määrati Leedu päritolu broileri kanalihal ja Eesti inimestelt pärinevatelt kampülobakterite isolaatidel, vastavalt 76,9% ja 80,0%. Streptomütsiiniresistentsete tüvede osakaal oli kõrge Läti ja Leedu päritolu broileriliha kampülobakterite isolaatide puhul, vastavalt 68,8% ja 42,3%. Multiresistentsete isolaatide proportsioon uuritud kampülobakterite isolaatide hulgas oli 26,2%. Kõik isolaadid olid tundlikud gentamütsiini suhtes. Erütromütsiini suhtes oli resistentsus madal ning kõik uuritud Eesti broileri kanaliha isolaadid olid tundlikud kõikide uuritud antibiootikumide suhtes. Leedu ja Läti päritolu kanabroilerilihast isoleeritud Campylobacter isolaatide ja kliiniliste (inimestelt isoleeritud) tüvede kõrge resistentsus uuritud antibiootikumide suhtes kujutab rahvatervise probleemi. Antimikroobse resistentsuse vähendamiseks peab antibiootikumide kasutamise kontroll broilerilihafarmi tasandil olema tõhusam ning antibiootikume ei tohi kasutada ennetavas ja metafülaktilises ravis, samuti kasvu soodustamise eesmärgil. | est |
| dc.identifier.uri | http://hdl.handle.net/10492/7573 | |
| dc.publisher | Eesti Maaülikool | |
| dc.subject | magistritööd | est |
| dc.subject | master thesis | eng |
| dc.subject | Campylobacter spp. | lat |
| dc.subject | antibiotic resistance | eng |
| dc.subject | fresh broiler chicken | eng |
| dc.subject | meat | eng |
| dc.subject | Green University (thesis is related to EMÜ Green University iniciative's aims) | eng |
| dc.subject | food safety | eng |
| dc.subject | organic food | eng |
| dc.title | Antimicrobial sensitivity of campylobacter spp. isolated from fresh broiler chicken meat of Estonian retail level | eng |
| dc.title.alternative | Eestis müüdavas värskes kanabroilerilihast isoleeritud Campylobacter spp. tundlikkus antimikroobsete ühendite suhtes | est |
| dc.type | Master Thesis | eng |
| rioxxterms.freetoread.startdate | 10.09.2022 |