Ultraheli meetodil liposoomse mikrokapsuleerimise seadme tehniline lahendus
Laen...
Kuupäev
2019
Kättesaadav alates
7.09.2019
Autorid
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Eesti Maaülikool
Abstrakt
Liposoomide kasutamise juures on üheks olulisemaks karakteristikuks nende läbimõõt.
Vajalik läbimõõt sõltub eelkõige liposoomide kasutusvaldkonnast. Ultrahelitöötlus on
väikeseläbimõõduliste liposoomide valmistamiseks üks kõige enam kasutatavaid meetodeid.
Tööstuslike ultrahelitöötluse seadmete kaasabil ultraheli otsese rakendamise meetodil on
liposoomide tekkimise dünaamikat kirjeldatud mitmetes varasemates teadusuuringutes.
Samas (käesoleva töö autorile teadaolevalt) ei ole kajastust leidnud kaudse ultrahelitöötluse
meetodi rakendamise põhjalikumad uuringud. Töö eesmärgiks on luua tehniline lahendus
erineva sageduse ja amplituudiga ultrahelitöötluse rakendamiseks letsitiini lahusele, et
tekitada võimalikult väikese läbimõõduga stabiilseid liposoome ning töötlemisega kaasneva
energiakulu määramine. Töö kirjutamisel on tuginetud kirjandusallikatele ja
teaduspublikatsioonidele ning töö läbiviimise käigus kogutud andmeid on võrreldud
varasemate läbiviidud uurimustega. Seadme juhtimiseks on koostatud vajalik tehniline
lahendus ning kontrollitud selle toimimist. Andmete statistilise olulisuse selgitamiseks ja
hüpoteeside kontrollimiseks kasutati t-testi. Töö tulemusel on jõutud järeldusele, et kaudse
töötlemisviisi efektiivsus on sarnane tööstuslike seadmete abil läbiviidud otsese töötlemise
käigus saadud tulemustega. Seadme juhtimiseks koostatud tehniline lahendus on toimiv ning
võimaldab jätkata uuringutega liposoomide valmistamismeetodi tõhusamaks muutmiseks.
Samuti on leitud seos lahuse kontsentratsiooni ja ultrahelitöötluse mõju vahel.
Application for usage of liposomes is largely dependent on the size of the liposomes. Sonication is widely used method for liposome size reduction. Various studies on the dynamics of liposome formation have been carried out with methods of direct sonication. Indirect sonication methods have not been examined enough. The aim of thesis is to develop a technical solution for the application of sonication with different frequencies and amplitudes and to study the effect of this on the process on liposome formation in lecithin solution and determination of the energy consumption associated with the treatment. Author has examined different sources of literature and scientific articles to describe and analyze methods for liposome production, size reduction and the effects of sonication. Data collected during the work were compared with previous works. Technical solution for operating conditions of the device has been constructed and several tests has been conducted. A statistical analyzes (student t-test) has been performed to test statistical significance of experiment results. It has been concluded that the effectiveness of the indirect treatment is comparable to that obtained by direct processing. The technical solution for controlling the device is workable and allows further research to make the method of producing liposomes more efficient. A relationship between solution concentration and sonication effect has also been found.
Application for usage of liposomes is largely dependent on the size of the liposomes. Sonication is widely used method for liposome size reduction. Various studies on the dynamics of liposome formation have been carried out with methods of direct sonication. Indirect sonication methods have not been examined enough. The aim of thesis is to develop a technical solution for the application of sonication with different frequencies and amplitudes and to study the effect of this on the process on liposome formation in lecithin solution and determination of the energy consumption associated with the treatment. Author has examined different sources of literature and scientific articles to describe and analyze methods for liposome production, size reduction and the effects of sonication. Data collected during the work were compared with previous works. Technical solution for operating conditions of the device has been constructed and several tests has been conducted. A statistical analyzes (student t-test) has been performed to test statistical significance of experiment results. It has been concluded that the effectiveness of the indirect treatment is comparable to that obtained by direct processing. The technical solution for controlling the device is workable and allows further research to make the method of producing liposomes more efficient. A relationship between solution concentration and sonication effect has also been found.
Kirjeldus
Magistritöö
Energiakasutuse õppekaval
Märksõnad
magistritööd, liposoomid, piesokeraamika, letitsiin, mikrokapsuleerimine, ultrahelitöötlus