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AFLP-analysis of genetic diversity in soybean [Glycine max (l.) Merr.] cultivars Russian and foreign selection

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dc.contributor.authorZargar, M.
dc.contributor.authorRomanova, E.
dc.contributor.authorTrifonova, A.
dc.contributor.authorShmelkova, E.
dc.contributor.authorKezimana, P.
dc.date.accessioned2017-10-25T06:52:18Z
dc.date.available2017-10-25T06:52:18Z
dc.date.issued2017
dc.descriptionArticleeng
dc.description.abstractGenetic diversity of 11 cultivars of soybean (Glycine max) from a VIR (N.I. Vavilov Institute of Plant Genetic Resources) collection was analyzed by the AFLP (amplified fragment length polymorphism) technique. From 18 tested primer combinations, both of them were selected for further analysis. From these two primer combinations (E32/M60 and E32/M59), 90 DNA fragments were obtained, 39 (43%) of them were polymorphic. Unique DNA fragments were found in accessions SibNIISHoz 6, Soer-4, Determinant. The Jaccard's similarity indices varied from 0.79 (between Sonata and Ugra) to 0.94 (between Ugra and Garmoniya) with an average of 0.775. Cluster analysis placed the cultivars into the several groups but separation in groups was not related to their origin or biological characteristics, even though cultivars Nordik and Soer-4 (cultivars recommended for Central Black Earth region of Russia) clustered together on the dendrogram. The obtained high values of Jaccard genetic similarity index and low level of polymorphism and differentiation illustrated a relatively low genetic diversity in our studied cultivars, which correlated with different other studies on soybean genetic diversity with AFLP-analysis.eng
dc.identifier.issn1406-894X
dc.identifier.publicationAgronomy Research, 2017, vol. 15, no. 5, pp. 2217-2225eng
dc.identifier.urihttp://hdl.handle.net/10492/3708
dc.identifier.urihttp://dx.doi.org/10.15159/ar.17.066
dc.rights.holderCopyright 2009 by Estonian University of Life Sciences, Latvia University of Agriculture, Aleksandras Stulginskis University, Lithuanian Research Centre for Agriculture and Forestry. No part of this publication may be reproduced or transmitted in any form, or by any means, electronic or mechanical, incl. photocopying, electronic recording, or otherwise without the prior written permission from the Estonian University of Life Sciences, Latvia University of Agriculture, Aleksandras Stulginskis University, Lithuanian Research Centre for Agriculture and Forestryeng
dc.subjectsoybeaneng
dc.subjectamplified fragment length polymorphism (AFLP)eng
dc.subjectgenetic diversityeng
dc.subjectmolecular markerseng
dc.subjectarticleseng
dc.titleAFLP-analysis of genetic diversity in soybean [Glycine max (l.) Merr.] cultivars Russian and foreign selectioneng
dc.typeArticleeng

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2017, Vol. 15, No. 5