AFLP-analysis of genetic diversity in soybean [Glycine max (l.) Merr.] cultivars Russian and foreign selection
Laen...
Kuupäev
2017
Kättesaadav alates
Autorid
Zargar, M.
Romanova, E.
Trifonova, A.
Shmelkova, E.
Kezimana, P.
Ajakirja pealkiri
Ajakirja ISSN
Köite pealkiri
Kirjastaja
Abstrakt
Genetic diversity of 11 cultivars of soybean (Glycine max) from a VIR (N.I. Vavilov
Institute of Plant Genetic Resources) collection was analyzed by the AFLP (amplified fragment
length polymorphism) technique. From 18 tested primer combinations, both of them were
selected for further analysis. From these two primer combinations (E32/M60 and E32/M59),
90 DNA fragments were obtained, 39 (43%) of them were polymorphic. Unique DNA fragments
were found in accessions SibNIISHoz 6, Soer-4, Determinant. The Jaccard's similarity indices
varied from 0.79 (between Sonata and Ugra) to 0.94 (between Ugra and Garmoniya) with an
average of 0.775. Cluster analysis placed the cultivars into the several groups but separation in
groups was not related to their origin or biological characteristics, even though cultivars Nordik
and Soer-4 (cultivars recommended for Central Black Earth region of Russia) clustered together
on the dendrogram. The obtained high values of Jaccard genetic similarity index and low level of
polymorphism and differentiation illustrated a relatively low genetic diversity in our studied
cultivars, which correlated with different other studies on soybean genetic diversity with
AFLP-analysis.
Kirjeldus
Article
Märksõnad
soybean, amplified fragment length polymorphism (AFLP), genetic diversity, molecular markers, articles