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Study of potential PCR inhibitors in drinking water for Escherichia coli identification

dc.contributor.authorDalecka, B.
dc.contributor.authorMezule, L.
dc.date.accessioned2018-05-09T10:47:56Z
dc.date.available2018-05-09T10:47:56Z
dc.date.issued2018
dc.descriptionArticleeng
dc.description.abstractIn the last few decades, the polymerase chain reaction (PCR) has become one of the most powerful molecular biological tools. However, the PCR is an enzymatic reaction and therefore sensitive to inhibitors which may occur in drinking water samples. In this work, the possible inhibition effect of chlorine, humic acids, and iron for real - time PCR (qPCR) efficiency was studied and the environmental sample from drinking water treatment system before iron removal was selected and analysed. The results demonstrate d that the highest concentrations of humic acids (5 mg L - 1 and 1 mg L - 1 ) and iron (4 mg L - 1 ) inhibited the PCR reaction while no effect of chlorine was observed. The analysis of the environmental sample with spiked Escherichia coli cells demonstrated reduc tion efficiency of the average threshold cycle (C t ) values compared with control dilution series determining the possible inhibition for qPCR assay.eng
dc.identifier.issn1406-894X
dc.identifier.publicationAgronomy Research, 2018, vol. 16, Special Issue 2, pp. 1351-1359eng
dc.identifier.urihttp://hdl.handle.net/10492/3944
dc.identifier.urihttp://dx.doi.org/10.15159/ar.18.118
dc.rights.holderCopyright 2009 by Estonian University of Life Sciences, Latvia University of Agriculture, Aleksandras Stulginskis University, Lithuanian Research Centre for Agriculture and Forestry. No part of this publication may be reproduced or transmitted in any form, or by any means, electronic or mechanical, incl. photocopying, electronic recording, or otherwise without the prior written permission from the Estonian University of Life Sciences, Latvia University of Agriculture, Aleksandras Stulginskis University, Lithuanian Research Centre for Agriculture and Forestryeng
dc.subjectpolymerase chain reactioneng
dc.subjectinhibitorseng
dc.subjectchlorineeng
dc.subjecthumic acidseng
dc.subjectironeng
dc.subjectEscherichia colieng
dc.subjectarticleseng
dc.titleStudy of potential PCR inhibitors in drinking water for Escherichia coli identificationeng
dc.typeArticleeng

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