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dc.contributor.authorBen-Othman, Sana
dc.contributor.authorRinken, Toonika
dc.date.accessioned2022-01-05T11:08:03Z
dc.date.available2022-01-05T11:08:03Z
dc.date.issued2021
dc.identifier.publicationAppl. Sci. 2021, 11, 4591eng
dc.identifier.issn2076-3417
dc.identifier.urihttp://hdl.handle.net/10492/7170
dc.identifier.urihttps://doi.org/10.3390/app11104591
dc.descriptionReceived: 27 April 2021; Accepted: 14 May 2021; Published: 18 May 2021.eng
dc.description.abstractPectinolytic enzymes are an important tool for sustainable food production, with a wide range of applications in food processing technologies as well as the extraction of bioactive compounds from pectin-rich raw materials. In the present study, we immobilized commercial pectinase preparation onto pellet and thread shaped nylon 6/6 carriers and assessed its stability and reusability. Five commercial pectinase preparations were tested for different pectin de-polymerizing activities (pectinase, polygalacturonase, and pectin lyase activities). Thereafter, Pectinex® Ultra Tropical preparation, exhibiting the highest catalytic activities among the studied preparations (p < 0.0001), was immobilized on nylon 6/6 using dimethyl sulfate and glutaraldehyde. The immobilization yield was in accordance with the carrier surface area available for enzyme attachment, and it was 1.25 0.10 U/g on threads, which was over 40 times higher than that on pellets. However, the inactivation of immobilized enzymes was not dependent on the shape of the carrier, indicating that the attachment of the enzymes on the surface of nylon 6/6 carriers was similar. The half-life of enzyme inactivation fast phase at 4 C was 12.8 days. After 5 weeks, the unused threads retained 63% of their initial activity. Reusability study showed that after 20 successive cycles the remaining activity of the immobilized pectinase was 22%, indicating the good prospects of reusability of the immobilized enzyme preparations for industrial application.eng
dc.description.abstractThis research is funded by VALORTECH project, which has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 810630.eng
dc.description.sponsorshipThis research is funded by VALORTECH project, which has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 810630.eng
dc.publisherMDPIeng
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0) ; openAccesseng
dc.rights© 2021 by the authors.eng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectpectinase immobilizationeng
dc.subjectnylon 6/6 carriereng
dc.subjectpectinolytic activityeng
dc.subjectreusabilityeng
dc.subjectstabilityeng
dc.subjectarticleseng
dc.titleImmobilization of Pectinolytic Enzymes on Nylon 6/6 Carrierseng
dc.typeArticleeng
dc.contributor.departmentERA Chair for Food (By-) Products Valorisation Technologies Valortech. Estonian University of Life Scienceseng
dc.identifier.citationBen-Othman, S.; Rinken, T. Immobilization of Pectinolytic Enzymes on Nylon 6/6 Carriers. Appl. Sci. 2021, 11, 4591. https:// doi.org/10.3390/app11104591eng


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Creative Commons Attribution 4.0 International (CC BY 4.0) ; openAccess
Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International (CC BY 4.0) ; openAccess